H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost

Aurin M. Vos, E. Jurak, Jordi F. Pelkmans, Koen Herman, Gill Pels, J.J.P. Baars, E.A.H.J. Hendrix, M.A. Kabel, Luis G. Lugones, Han A.B. Wösten

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Degradation of lignin by fungi enhances availability of cellulose and hemicellulose in plant waste and thereby increases the amount of carbon source available to these microorganisms. The button mushroom Agaricus bisporus
degrades only about half of the lignin in compost and about 40% of the carbohydrates remain unutilized during mushroom cultivation. Here it was assessed whether over-expression of the manganese peroxidase gene mnp1
improves lignin degradation and, as a consequence, carbohydrate breakdown by A. bisporus. Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while the
parental strain A15 did not. MnP activity was increased 0.3- and 3-fold at casing and after the 2nd flush of a semicommercial cultivation, respectively, when compared to strain A15. Pyrolysis-GC–MS showed that overexpression of
MnP decreased phenylmethane and phenylethane type lignin relative to the phenylpropane type after the 2nd flush.
However, it neither affected the syringyl/guaiacyl derived residue ratio nor the ratio of oxidized to non-oxidized lignin residues. Moreover, the carbohydrate content and accessibility was not affected in compost. Notably, the capacity
of compost extract to consume the MnP co-factor H2O2 was 4- to 8-fold higher than its production. This may well explain why over-expression of mnp1 did not improve carbohydrate degradation in compost. In fact, availability of
H2O2 may limit lignin degradation by wild-type A. bisporus.
Original languageEnglish
Article number124
JournalAMB Express
Volume7
DOIs
Publication statusPublished - 2017

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Agaricus
Lignin
Soil
Carbohydrates
Agaricales
manganese peroxidase
Cellulose
Gas Chromatography-Mass Spectrometry
Actins
Fungi
Carbon
Genes

Cite this

Vos, Aurin M. ; Jurak, E. ; Pelkmans, Jordi F. ; Herman, Koen ; Pels, Gill ; Baars, J.J.P. ; Hendrix, E.A.H.J. ; Kabel, M.A. ; Lugones, Luis G. ; Wösten, Han A.B. / H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost. In: AMB Express. 2017 ; Vol. 7.
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title = "H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost",
abstract = "Degradation of lignin by fungi enhances availability of cellulose and hemicellulose in plant waste and thereby increases the amount of carbon source available to these microorganisms. The button mushroom Agaricus bisporusdegrades only about half of the lignin in compost and about 40{\%} of the carbohydrates remain unutilized during mushroom cultivation. Here it was assessed whether over-expression of the manganese peroxidase gene mnp1improves lignin degradation and, as a consequence, carbohydrate breakdown by A. bisporus. Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while theparental strain A15 did not. MnP activity was increased 0.3- and 3-fold at casing and after the 2nd flush of a semicommercial cultivation, respectively, when compared to strain A15. Pyrolysis-GC–MS showed that overexpression ofMnP decreased phenylmethane and phenylethane type lignin relative to the phenylpropane type after the 2nd flush.However, it neither affected the syringyl/guaiacyl derived residue ratio nor the ratio of oxidized to non-oxidized lignin residues. Moreover, the carbohydrate content and accessibility was not affected in compost. Notably, the capacityof compost extract to consume the MnP co-factor H2O2 was 4- to 8-fold higher than its production. This may well explain why over-expression of mnp1 did not improve carbohydrate degradation in compost. In fact, availability ofH2O2 may limit lignin degradation by wild-type A. bisporus.",
author = "Vos, {Aurin M.} and E. Jurak and Pelkmans, {Jordi F.} and Koen Herman and Gill Pels and J.J.P. Baars and E.A.H.J. Hendrix and M.A. Kabel and Lugones, {Luis G.} and W{\"o}sten, {Han A.B.}",
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language = "English",
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H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost. / Vos, Aurin M.; Jurak, E.; Pelkmans, Jordi F.; Herman, Koen; Pels, Gill; Baars, J.J.P.; Hendrix, E.A.H.J.; Kabel, M.A.; Lugones, Luis G.; Wösten, Han A.B.

In: AMB Express, Vol. 7, 124, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost

AU - Vos, Aurin M.

AU - Jurak, E.

AU - Pelkmans, Jordi F.

AU - Herman, Koen

AU - Pels, Gill

AU - Baars, J.J.P.

AU - Hendrix, E.A.H.J.

AU - Kabel, M.A.

AU - Lugones, Luis G.

AU - Wösten, Han A.B.

PY - 2017

Y1 - 2017

N2 - Degradation of lignin by fungi enhances availability of cellulose and hemicellulose in plant waste and thereby increases the amount of carbon source available to these microorganisms. The button mushroom Agaricus bisporusdegrades only about half of the lignin in compost and about 40% of the carbohydrates remain unutilized during mushroom cultivation. Here it was assessed whether over-expression of the manganese peroxidase gene mnp1improves lignin degradation and, as a consequence, carbohydrate breakdown by A. bisporus. Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while theparental strain A15 did not. MnP activity was increased 0.3- and 3-fold at casing and after the 2nd flush of a semicommercial cultivation, respectively, when compared to strain A15. Pyrolysis-GC–MS showed that overexpression ofMnP decreased phenylmethane and phenylethane type lignin relative to the phenylpropane type after the 2nd flush.However, it neither affected the syringyl/guaiacyl derived residue ratio nor the ratio of oxidized to non-oxidized lignin residues. Moreover, the carbohydrate content and accessibility was not affected in compost. Notably, the capacityof compost extract to consume the MnP co-factor H2O2 was 4- to 8-fold higher than its production. This may well explain why over-expression of mnp1 did not improve carbohydrate degradation in compost. In fact, availability ofH2O2 may limit lignin degradation by wild-type A. bisporus.

AB - Degradation of lignin by fungi enhances availability of cellulose and hemicellulose in plant waste and thereby increases the amount of carbon source available to these microorganisms. The button mushroom Agaricus bisporusdegrades only about half of the lignin in compost and about 40% of the carbohydrates remain unutilized during mushroom cultivation. Here it was assessed whether over-expression of the manganese peroxidase gene mnp1improves lignin degradation and, as a consequence, carbohydrate breakdown by A. bisporus. Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while theparental strain A15 did not. MnP activity was increased 0.3- and 3-fold at casing and after the 2nd flush of a semicommercial cultivation, respectively, when compared to strain A15. Pyrolysis-GC–MS showed that overexpression ofMnP decreased phenylmethane and phenylethane type lignin relative to the phenylpropane type after the 2nd flush.However, it neither affected the syringyl/guaiacyl derived residue ratio nor the ratio of oxidized to non-oxidized lignin residues. Moreover, the carbohydrate content and accessibility was not affected in compost. Notably, the capacityof compost extract to consume the MnP co-factor H2O2 was 4- to 8-fold higher than its production. This may well explain why over-expression of mnp1 did not improve carbohydrate degradation in compost. In fact, availability ofH2O2 may limit lignin degradation by wild-type A. bisporus.

UR - https://doi.org/10.6084/m9.figshare.c.3805495

U2 - 10.1186/s13568-017-0424-z

DO - 10.1186/s13568-017-0424-z

M3 - Article

VL - 7

JO - AMB Express

JF - AMB Express

SN - 2191-0855

M1 - 124

ER -