Growth of Ca-D-malate crystals in a bioreactor

M.J.F. Michielsen, C. Frielink, R.H. Wijffels, J. Tramper, H.H. Beeftink

    Research output: Contribution to journalArticleAcademicpeer-review

    4 Citations (Scopus)


    To develop a bioreactor for solid-to-solid conversions, the conversion of solid Ca-maleate to solid Ca-D-malate by permeabilized Pseudomonas pseudoalcaligenes was studied. In a bioreactor seeded with product (Ca-D-malate) crystals, growth of Ca-D-malate crystals is the last step in the solid-to-solid conversion and is described here. Crystal growth is described as a transport process followed by surface processes. In contrast to the linear rate law obeyed by the transport process, the surface processes of a crystal-growth process can also obey a parabolic or exponential rate law. Growth of Ca-D-malate crystals from a supersaturated aqueous solution was found to be surface-controlled and obeyed an exponential rate law. Based on this rate law, a kinetic model was developed which describes the decrease in supersaturation due to Ca-D-malate crystal growth as a function of the constituent ions, Ca2 and D-malate2-. The kinetic parameters depended on temperature, but, as expected (surface-controlled), they were hardly affected by the stirring speed
    Original languageEnglish
    Pages (from-to)548-558
    JournalBiotechnology and Bioengineering
    Publication statusPublished - 2000

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