Growth and morphogenesis of shoot initials of Douglas fir, Pseudotsuga menziesii (Mirb.) Franco, in vitro

P.W. Evers

    Research output: Thesisinternal PhD, WU

    Abstract

    An optimalized method of micropropagation of Douglas fir is described. Seasonal changes were found in optima for nitrate and sucrose in the medium and in the optimum for the light intensity during the culture of shoot initials. Differences in morphogenesis were obtained from shoot initials that had been isolated from buds in 10 topophysical positions on 2-year-old trees. These differences between the shoots were influenced by the medium, the light intensity, and by forcing or topping the mother trees or treating them with growth regulators. Shoots from each of the topophysical positions had a characteristic photosynthesis. A first attempt is made to compare in vivo flushing with the morphogenesis of shoot initials in vitro.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    Supervisors/Advisors
    • Oldeman, R.A.A., Promotor
    • Pierik, R.L.M., Co-promotor
    Award date14 Mar 1984
    Place of PublicationWageningen
    Publisher
    Publication statusPublished - 1984

    Keywords

    • asexual reproduction
    • buds
    • environmental factors
    • forestry
    • forestry practices
    • growth
    • in vitro culture
    • plant morphology
    • shoots
    • silviculture
    • stems
    • trees
    • vegetative propagation
    • pseudotsuga menziesii

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