Green light for quantitative live-cell imaging in plants

Guido Grossmann, Melanie Krebs, Alexis Maizel, Yvonne Stahl, Joop E.M. Vermeer, Thomas Ott*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Plants exhibit an intriguing morphological and physiological plasticity that enables them to thrive in a wide range of environments. To understand the cell biological basis of this unparalleled competence, a number ofmethodologies have been adapted or developed over the last decades that allow minimal or non-invasive live-cell imaging in the context of tissues. Combined with the ease to generate transgenic reporter lines in specific genetic backgrounds or accessions, we are witnessing a blooming in plant cell biology. However, the imaging of plant cells entails a number of specific challenges, such as high levels of autofluorescence, light scattering that is caused by cell walls and their sensitivity to environmental conditions. Quantitative live-cell imaging in plants therefore requires adapting or developing imaging techniques, as well as mounting and incubation systems, such as micro-fluidics. Here, we discuss some of these obstacles, and review a number of selected state-of-the-art techniques, such as two-photon imaging, light sheet microscopy and variable angle epifluorescence microscopy that allow high performance and minimal invasive live-cell imaging in plants.
Original languageEnglish
Article numberjcs.209270
JournalJournal of Cell Science
Volume131
Issue number2
DOIs
Publication statusPublished - 29 Jan 2018

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Methyl Green
Plant Cells
Microscopy
Light
Photons
Mental Competency
Cell Wall
Cell Biology

Keywords

  • Imaging
  • Plant cell biology
  • Plant growth

Cite this

Grossmann, G., Krebs, M., Maizel, A., Stahl, Y., Vermeer, J. E. M., & Ott, T. (2018). Green light for quantitative live-cell imaging in plants. Journal of Cell Science, 131(2), [jcs.209270]. https://doi.org/10.1242/jcs.209270
Grossmann, Guido ; Krebs, Melanie ; Maizel, Alexis ; Stahl, Yvonne ; Vermeer, Joop E.M. ; Ott, Thomas. / Green light for quantitative live-cell imaging in plants. In: Journal of Cell Science. 2018 ; Vol. 131, No. 2.
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Grossmann, G, Krebs, M, Maizel, A, Stahl, Y, Vermeer, JEM & Ott, T 2018, 'Green light for quantitative live-cell imaging in plants', Journal of Cell Science, vol. 131, no. 2, jcs.209270. https://doi.org/10.1242/jcs.209270

Green light for quantitative live-cell imaging in plants. / Grossmann, Guido; Krebs, Melanie; Maizel, Alexis; Stahl, Yvonne; Vermeer, Joop E.M.; Ott, Thomas.

In: Journal of Cell Science, Vol. 131, No. 2, jcs.209270, 29.01.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Ott, Thomas

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Grossmann G, Krebs M, Maizel A, Stahl Y, Vermeer JEM, Ott T. Green light for quantitative live-cell imaging in plants. Journal of Cell Science. 2018 Jan 29;131(2). jcs.209270. https://doi.org/10.1242/jcs.209270