Green compressed fluid technologies for downstream processing of Scenedesmus obliquus in a biorefinery approach

Bienvenida Gilbert-López, José A. Mendiola, Lambertus A.M. van den Broek, Bwee Houweling-Tan, Lolke Sijtsma, Alejandro Cifuentes, Miguel Herrero, Elena Ibáñez*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

The fractionation of algae biomass in several high-value compounds that can be used as ingredients in other applications sets the basis of the algae biorefinery approach. The present study aimed at the extraction and fractionation of bioactive compounds from the microalga Scenedesmus obliquus, by means of applying a sequential process without the manipulation of the biomass in the extraction cell. This integrated platform of compressed fluid extraction technologies of low-environmental impact was designed in order to produce increases of solvent polarity using non-toxic solvents. The process involved the following steps:(1) supercritical fluid extraction (SFE) using supercritical carbon dioxide (ScCO2); (2) gas expanded liquids (GXL) using 75% ethanol and 25% ScCO2 (v/v) and; (3) pressurized liquid extraction (PLE) using water. Extraction conditions were optimized using response surface methodology (RSM) and kinetic studies. Extraction yield, antioxidant activity as well as contents of total phenols, carotenoids, proteins and sugars were the studied response variables. High performance liquid chromatography coupled to evaporative light-scattering detector (HPLC-ELSD) analyses of the fractions revealed that triacylglycerols were mainly extracted by SFE. Lutein and β-carotene were the main pigments identified in the extracts by HPLC coupled to diode array and mass spectrometry detectors (HPLC-DAD-MS/MS), which were preferentially extracted in the GXL step. Polar compounds such as proteins and sugars remained predominantly in the residue. Therefore, the green downstream platform developed in this study for valorization of the microalgae biomass, is able to produce different fractions with potential application in the food, pharmaceutical and cosmetic industries.

Original languageEnglish
Pages (from-to)111-121
JournalAlgal Research
Volume24
DOIs
Publication statusPublished - 1 Jun 2017

Fingerprint

Scenedesmus obliquus
biorefining
algae
supercritical fluid extraction
high performance liquid chromatography
detectors
liquids
biomass
fractionation
gases
sugars
polar compounds
light scattering
carotenes
cosmetics
response surface methodology
lutein
microalgae
phenols
fluids

Keywords

  • Algae
  • Biorefinery
  • Carotenoid
  • Lipid
  • Pressurized liquid extraction
  • Supercritical fluid extraction

Cite this

Gilbert-López, Bienvenida ; Mendiola, José A. ; van den Broek, Lambertus A.M. ; Houweling-Tan, Bwee ; Sijtsma, Lolke ; Cifuentes, Alejandro ; Herrero, Miguel ; Ibáñez, Elena. / Green compressed fluid technologies for downstream processing of Scenedesmus obliquus in a biorefinery approach. In: Algal Research. 2017 ; Vol. 24. pp. 111-121.
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abstract = "The fractionation of algae biomass in several high-value compounds that can be used as ingredients in other applications sets the basis of the algae biorefinery approach. The present study aimed at the extraction and fractionation of bioactive compounds from the microalga Scenedesmus obliquus, by means of applying a sequential process without the manipulation of the biomass in the extraction cell. This integrated platform of compressed fluid extraction technologies of low-environmental impact was designed in order to produce increases of solvent polarity using non-toxic solvents. The process involved the following steps:(1) supercritical fluid extraction (SFE) using supercritical carbon dioxide (ScCO2); (2) gas expanded liquids (GXL) using 75{\%} ethanol and 25{\%} ScCO2 (v/v) and; (3) pressurized liquid extraction (PLE) using water. Extraction conditions were optimized using response surface methodology (RSM) and kinetic studies. Extraction yield, antioxidant activity as well as contents of total phenols, carotenoids, proteins and sugars were the studied response variables. High performance liquid chromatography coupled to evaporative light-scattering detector (HPLC-ELSD) analyses of the fractions revealed that triacylglycerols were mainly extracted by SFE. Lutein and β-carotene were the main pigments identified in the extracts by HPLC coupled to diode array and mass spectrometry detectors (HPLC-DAD-MS/MS), which were preferentially extracted in the GXL step. Polar compounds such as proteins and sugars remained predominantly in the residue. Therefore, the green downstream platform developed in this study for valorization of the microalgae biomass, is able to produce different fractions with potential application in the food, pharmaceutical and cosmetic industries.",
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author = "Bienvenida Gilbert-L{\'o}pez and Mendiola, {Jos{\'e} A.} and {van den Broek}, {Lambertus A.M.} and Bwee Houweling-Tan and Lolke Sijtsma and Alejandro Cifuentes and Miguel Herrero and Elena Ib{\'a}{\~n}ez",
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Green compressed fluid technologies for downstream processing of Scenedesmus obliquus in a biorefinery approach. / Gilbert-López, Bienvenida; Mendiola, José A.; van den Broek, Lambertus A.M.; Houweling-Tan, Bwee; Sijtsma, Lolke; Cifuentes, Alejandro; Herrero, Miguel; Ibáñez, Elena.

In: Algal Research, Vol. 24, 01.06.2017, p. 111-121.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Green compressed fluid technologies for downstream processing of Scenedesmus obliquus in a biorefinery approach

AU - Gilbert-López, Bienvenida

AU - Mendiola, José A.

AU - van den Broek, Lambertus A.M.

AU - Houweling-Tan, Bwee

AU - Sijtsma, Lolke

AU - Cifuentes, Alejandro

AU - Herrero, Miguel

AU - Ibáñez, Elena

PY - 2017/6/1

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N2 - The fractionation of algae biomass in several high-value compounds that can be used as ingredients in other applications sets the basis of the algae biorefinery approach. The present study aimed at the extraction and fractionation of bioactive compounds from the microalga Scenedesmus obliquus, by means of applying a sequential process without the manipulation of the biomass in the extraction cell. This integrated platform of compressed fluid extraction technologies of low-environmental impact was designed in order to produce increases of solvent polarity using non-toxic solvents. The process involved the following steps:(1) supercritical fluid extraction (SFE) using supercritical carbon dioxide (ScCO2); (2) gas expanded liquids (GXL) using 75% ethanol and 25% ScCO2 (v/v) and; (3) pressurized liquid extraction (PLE) using water. Extraction conditions were optimized using response surface methodology (RSM) and kinetic studies. Extraction yield, antioxidant activity as well as contents of total phenols, carotenoids, proteins and sugars were the studied response variables. High performance liquid chromatography coupled to evaporative light-scattering detector (HPLC-ELSD) analyses of the fractions revealed that triacylglycerols were mainly extracted by SFE. Lutein and β-carotene were the main pigments identified in the extracts by HPLC coupled to diode array and mass spectrometry detectors (HPLC-DAD-MS/MS), which were preferentially extracted in the GXL step. Polar compounds such as proteins and sugars remained predominantly in the residue. Therefore, the green downstream platform developed in this study for valorization of the microalgae biomass, is able to produce different fractions with potential application in the food, pharmaceutical and cosmetic industries.

AB - The fractionation of algae biomass in several high-value compounds that can be used as ingredients in other applications sets the basis of the algae biorefinery approach. The present study aimed at the extraction and fractionation of bioactive compounds from the microalga Scenedesmus obliquus, by means of applying a sequential process without the manipulation of the biomass in the extraction cell. This integrated platform of compressed fluid extraction technologies of low-environmental impact was designed in order to produce increases of solvent polarity using non-toxic solvents. The process involved the following steps:(1) supercritical fluid extraction (SFE) using supercritical carbon dioxide (ScCO2); (2) gas expanded liquids (GXL) using 75% ethanol and 25% ScCO2 (v/v) and; (3) pressurized liquid extraction (PLE) using water. Extraction conditions were optimized using response surface methodology (RSM) and kinetic studies. Extraction yield, antioxidant activity as well as contents of total phenols, carotenoids, proteins and sugars were the studied response variables. High performance liquid chromatography coupled to evaporative light-scattering detector (HPLC-ELSD) analyses of the fractions revealed that triacylglycerols were mainly extracted by SFE. Lutein and β-carotene were the main pigments identified in the extracts by HPLC coupled to diode array and mass spectrometry detectors (HPLC-DAD-MS/MS), which were preferentially extracted in the GXL step. Polar compounds such as proteins and sugars remained predominantly in the residue. Therefore, the green downstream platform developed in this study for valorization of the microalgae biomass, is able to produce different fractions with potential application in the food, pharmaceutical and cosmetic industries.

KW - Algae

KW - Biorefinery

KW - Carotenoid

KW - Lipid

KW - Pressurized liquid extraction

KW - Supercritical fluid extraction

U2 - 10.1016/j.algal.2017.03.011

DO - 10.1016/j.algal.2017.03.011

M3 - Article

VL - 24

SP - 111

EP - 121

JO - Algal Research

JF - Algal Research

SN - 2211-9264

ER -