Granulomatous enteritis in rainbow trout (Oncorhynchus mykiss) associated with soya bean meal regardless of water dissolved oxygen level

P. Mosberian-Tanha, T. Landsverk, C.M. Press, L.T. Mydland, J.W. Schrama, M. Øverland*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

This study investigated morphological changes associated with soya bean meal-induced enteritis (SBMIE) in distal intestine (DI) of rainbow trout (Oncorhynchus mykiss) fed a soya bean meal (SBM)-based diet and exposed to normoxia or hypoxia created by optimal and low water flow rates, respectively. A 28-day adaption period was followed by a 42-day challenge period where 600 fish were subjected to dietary challenge and/or hypoxia. Twelve tanks each containing 50 juvenile trout were assigned randomly in triplicate to each treatment. Histopathological and immunohistochemical evaluation revealed pathological features that have not previously been described in association with SBMIE. Vacuolar degeneration of epithelial cells mainly at the base of mucosal folds, epithelial cysts, epithelial dysplasia, necrosis, shedding of necrotic cells, and granulomatous inflammation including infiltration of enlarged, sometimes finely vacuolated or “foamy” macrophages, multinucleated giant cells and increased proliferation of fibroblasts were observed. Acid-fast bacteria were not detected in enlarged macrophages; however, these cells contained AB-PAS- and sometimes cytokeratin-positive material, which was interpreted to be of epithelial/goblet cell origin. Hypoxia did not affect the morphological changes in DI. These results suggest that SBM was associated with a granulomatous form of enteritis in DI of rainbow trout regardless of water oxygen level.
Original languageEnglish
Pages (from-to)269-280
JournalJournal of Fish Diseases
Volume41
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Oncorhynchus mykiss
enteritis
hypoxia
Crohn Disease
dissolved oxygen
rainbow
soybean meal
Meals
Oxygen
Intestines
intestines
Enteritis
Water
macrophages
epithelial cells
water
Epithelial Cells
Macrophages
cyst
low flow

Keywords

  • foamy macrophages
  • granulomatous enteritis
  • hypoxia
  • rainbow trout
  • soya bean meal

Cite this

Mosberian-Tanha, P. ; Landsverk, T. ; Press, C.M. ; Mydland, L.T. ; Schrama, J.W. ; Øverland, M. / Granulomatous enteritis in rainbow trout (Oncorhynchus mykiss) associated with soya bean meal regardless of water dissolved oxygen level. In: Journal of Fish Diseases. 2018 ; Vol. 41, No. 2. pp. 269-280.
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abstract = "This study investigated morphological changes associated with soya bean meal-induced enteritis (SBMIE) in distal intestine (DI) of rainbow trout (Oncorhynchus mykiss) fed a soya bean meal (SBM)-based diet and exposed to normoxia or hypoxia created by optimal and low water flow rates, respectively. A 28-day adaption period was followed by a 42-day challenge period where 600 fish were subjected to dietary challenge and/or hypoxia. Twelve tanks each containing 50 juvenile trout were assigned randomly in triplicate to each treatment. Histopathological and immunohistochemical evaluation revealed pathological features that have not previously been described in association with SBMIE. Vacuolar degeneration of epithelial cells mainly at the base of mucosal folds, epithelial cysts, epithelial dysplasia, necrosis, shedding of necrotic cells, and granulomatous inflammation including infiltration of enlarged, sometimes finely vacuolated or “foamy” macrophages, multinucleated giant cells and increased proliferation of fibroblasts were observed. Acid-fast bacteria were not detected in enlarged macrophages; however, these cells contained AB-PAS- and sometimes cytokeratin-positive material, which was interpreted to be of epithelial/goblet cell origin. Hypoxia did not affect the morphological changes in DI. These results suggest that SBM was associated with a granulomatous form of enteritis in DI of rainbow trout regardless of water oxygen level.",
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Granulomatous enteritis in rainbow trout (Oncorhynchus mykiss) associated with soya bean meal regardless of water dissolved oxygen level. / Mosberian-Tanha, P.; Landsverk, T.; Press, C.M.; Mydland, L.T.; Schrama, J.W.; Øverland, M.

In: Journal of Fish Diseases, Vol. 41, No. 2, 01.02.2018, p. 269-280.

Research output: Contribution to journalArticleAcademicpeer-review

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