Granulocyte-macrophage colony-stimulating factor negatively regulates early IL-10-mediated responses

Ruud H.P. Wilbers, Lotte B. Westerhof, Debbie R. Van Raaij, Jaap Bakker, Geert Smant, Arjen Schots

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aim: Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis. IL-10 is a cytokine with therapeutic potential, but until now has not been as successful as previously anticipated. A reason for this may be that IL-10 responsiveness depends on the environment of the inflamed tissue. In this study we investigated whether GM-CSF is able to influence IL-10-mediated responses. Methodology: Dendritic cells and macrophages were differentiated from mouse bone marrow and treated or depleted from GM-CSF prior to analyze their response to IL-10. Activity was assessed by measuring cytokine expression upon lipopolysaccharide stimulation, IL-10-induced signaling and downstream gene expression. Conclusion: This study describes that GM-CSF negatively regulates IL-10-mediated responses.
Original languageEnglish
Article number10.4155
JournalFuture Science OA
Volume14
Issue number4
Early online date14 Feb 2018
DOIs
Publication statusPublished - Apr 2018

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Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-10
Cytokines
Dendritic Cells
Lipopolysaccharides
Homeostasis
Bone Marrow
Macrophages
Gene Expression

Cite this

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title = "Granulocyte-macrophage colony-stimulating factor negatively regulates early IL-10-mediated responses",
abstract = "Aim: Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis. IL-10 is a cytokine with therapeutic potential, but until now has not been as successful as previously anticipated. A reason for this may be that IL-10 responsiveness depends on the environment of the inflamed tissue. In this study we investigated whether GM-CSF is able to influence IL-10-mediated responses. Methodology: Dendritic cells and macrophages were differentiated from mouse bone marrow and treated or depleted from GM-CSF prior to analyze their response to IL-10. Activity was assessed by measuring cytokine expression upon lipopolysaccharide stimulation, IL-10-induced signaling and downstream gene expression. Conclusion: This study describes that GM-CSF negatively regulates IL-10-mediated responses.",
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Granulocyte-macrophage colony-stimulating factor negatively regulates early IL-10-mediated responses. / Wilbers, Ruud H.P.; Westerhof, Lotte B.; Van Raaij, Debbie R.; Bakker, Jaap; Smant, Geert; Schots, Arjen.

In: Future Science OA, Vol. 14, No. 4, 10.4155, 04.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Granulocyte-macrophage colony-stimulating factor negatively regulates early IL-10-mediated responses

AU - Wilbers, Ruud H.P.

AU - Westerhof, Lotte B.

AU - Van Raaij, Debbie R.

AU - Bakker, Jaap

AU - Smant, Geert

AU - Schots, Arjen

PY - 2018/4

Y1 - 2018/4

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AB - Aim: Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis. IL-10 is a cytokine with therapeutic potential, but until now has not been as successful as previously anticipated. A reason for this may be that IL-10 responsiveness depends on the environment of the inflamed tissue. In this study we investigated whether GM-CSF is able to influence IL-10-mediated responses. Methodology: Dendritic cells and macrophages were differentiated from mouse bone marrow and treated or depleted from GM-CSF prior to analyze their response to IL-10. Activity was assessed by measuring cytokine expression upon lipopolysaccharide stimulation, IL-10-induced signaling and downstream gene expression. Conclusion: This study describes that GM-CSF negatively regulates IL-10-mediated responses.

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