Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

It is claimed that bifidobacteria have several health-promoting effects. To increase the amount of bifidobacteria in the colon the concept of probiotics and/or prebiotics can be applied. Bifidobacterium adolescentis is one of the main species of bifidobacteria in the gastro-intestinal tract of human adults. B. adolescentis is able to degrade a wide range of oligosaccharides and a number of glycosyl hydrolases have been characterized in detail. The hydrolytic activity of the glycosyl hydrolases from B. adolescentis toward prebiotics like arabinoxylan-oligosaccharides, isomalto-oligosaccharides, arabinogalactan, and sucrose-based oligosaccharides (raffinose, stachyose, and fructo- oligosaccharides) is reviewed. Alternatively, some of these glycosyl hydrolases are able to catalyze transglycosylation, which allows them to elongate oligosaccharides and to prepare potentially prebiotic oligosaccharides. Such oligo saccharides might be used to influence the microbial composition in the more distal parts of the colon. In nature, not all enzyme-substrate encounters are transglycosylating. So, the hydrolytic activity of the enzyme makes the oligosaccharide elongation less efficient than desired. Site-directed mutagenesis was applied to improve the transglycosylation reaction of the ¿-galactosidase from B. adolescentis.
Original languageEnglish
Pages (from-to)125-133
JournalLait
Volume85
Issue number1-2
DOIs
Publication statusPublished - 2005

Fingerprint

Bifidobacterium adolescentis
glycosidases
Hydrolases
Oligosaccharides
oligosaccharides
Prebiotics
Bifidobacterium
prebiotics
colon
Colon
galactosidases
arabinogalactans
Galactosidases
stachyose
health promotion
arabinoxylan
Raffinose
fructooligosaccharides
enzyme substrates
raffinose

Keywords

  • alpha-galactosidase
  • dsm 20083
  • catalytic nucleophile
  • sucrose phosphorylase
  • beta-galactosidases
  • genome sequence
  • active-center
  • oligosaccharides
  • prebiotics
  • probiotics

Cite this

@article{b04ff0bade7d4aae8bd1bee659b0f688,
title = "Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview",
abstract = "It is claimed that bifidobacteria have several health-promoting effects. To increase the amount of bifidobacteria in the colon the concept of probiotics and/or prebiotics can be applied. Bifidobacterium adolescentis is one of the main species of bifidobacteria in the gastro-intestinal tract of human adults. B. adolescentis is able to degrade a wide range of oligosaccharides and a number of glycosyl hydrolases have been characterized in detail. The hydrolytic activity of the glycosyl hydrolases from B. adolescentis toward prebiotics like arabinoxylan-oligosaccharides, isomalto-oligosaccharides, arabinogalactan, and sucrose-based oligosaccharides (raffinose, stachyose, and fructo- oligosaccharides) is reviewed. Alternatively, some of these glycosyl hydrolases are able to catalyze transglycosylation, which allows them to elongate oligosaccharides and to prepare potentially prebiotic oligosaccharides. Such oligo saccharides might be used to influence the microbial composition in the more distal parts of the colon. In nature, not all enzyme-substrate encounters are transglycosylating. So, the hydrolytic activity of the enzyme makes the oligosaccharide elongation less efficient than desired. Site-directed mutagenesis was applied to improve the transglycosylation reaction of the ¿-galactosidase from B. adolescentis.",
keywords = "alpha-galactosidase, dsm 20083, catalytic nucleophile, sucrose phosphorylase, beta-galactosidases, genome sequence, active-center, oligosaccharides, prebiotics, probiotics",
author = "{van den Broek}, L.A.M. and S.W.A. Hinz and G. Beldman and C.H.L. Doeswijk-Voragen and J.P. Vincken and A.G.J. Voragen",
year = "2005",
doi = "10.1051/lait:2005004",
language = "English",
volume = "85",
pages = "125--133",
journal = "Lait",
issn = "0023-7302",
publisher = "EDP Sciences",
number = "1-2",

}

Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview. / van den Broek, L.A.M.; Hinz, S.W.A.; Beldman, G.; Doeswijk-Voragen, C.H.L.; Vincken, J.P.; Voragen, A.G.J.

In: Lait, Vol. 85, No. 1-2, 2005, p. 125-133.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview

AU - van den Broek, L.A.M.

AU - Hinz, S.W.A.

AU - Beldman, G.

AU - Doeswijk-Voragen, C.H.L.

AU - Vincken, J.P.

AU - Voragen, A.G.J.

PY - 2005

Y1 - 2005

N2 - It is claimed that bifidobacteria have several health-promoting effects. To increase the amount of bifidobacteria in the colon the concept of probiotics and/or prebiotics can be applied. Bifidobacterium adolescentis is one of the main species of bifidobacteria in the gastro-intestinal tract of human adults. B. adolescentis is able to degrade a wide range of oligosaccharides and a number of glycosyl hydrolases have been characterized in detail. The hydrolytic activity of the glycosyl hydrolases from B. adolescentis toward prebiotics like arabinoxylan-oligosaccharides, isomalto-oligosaccharides, arabinogalactan, and sucrose-based oligosaccharides (raffinose, stachyose, and fructo- oligosaccharides) is reviewed. Alternatively, some of these glycosyl hydrolases are able to catalyze transglycosylation, which allows them to elongate oligosaccharides and to prepare potentially prebiotic oligosaccharides. Such oligo saccharides might be used to influence the microbial composition in the more distal parts of the colon. In nature, not all enzyme-substrate encounters are transglycosylating. So, the hydrolytic activity of the enzyme makes the oligosaccharide elongation less efficient than desired. Site-directed mutagenesis was applied to improve the transglycosylation reaction of the ¿-galactosidase from B. adolescentis.

AB - It is claimed that bifidobacteria have several health-promoting effects. To increase the amount of bifidobacteria in the colon the concept of probiotics and/or prebiotics can be applied. Bifidobacterium adolescentis is one of the main species of bifidobacteria in the gastro-intestinal tract of human adults. B. adolescentis is able to degrade a wide range of oligosaccharides and a number of glycosyl hydrolases have been characterized in detail. The hydrolytic activity of the glycosyl hydrolases from B. adolescentis toward prebiotics like arabinoxylan-oligosaccharides, isomalto-oligosaccharides, arabinogalactan, and sucrose-based oligosaccharides (raffinose, stachyose, and fructo- oligosaccharides) is reviewed. Alternatively, some of these glycosyl hydrolases are able to catalyze transglycosylation, which allows them to elongate oligosaccharides and to prepare potentially prebiotic oligosaccharides. Such oligo saccharides might be used to influence the microbial composition in the more distal parts of the colon. In nature, not all enzyme-substrate encounters are transglycosylating. So, the hydrolytic activity of the enzyme makes the oligosaccharide elongation less efficient than desired. Site-directed mutagenesis was applied to improve the transglycosylation reaction of the ¿-galactosidase from B. adolescentis.

KW - alpha-galactosidase

KW - dsm 20083

KW - catalytic nucleophile

KW - sucrose phosphorylase

KW - beta-galactosidases

KW - genome sequence

KW - active-center

KW - oligosaccharides

KW - prebiotics

KW - probiotics

U2 - 10.1051/lait:2005004

DO - 10.1051/lait:2005004

M3 - Article

VL - 85

SP - 125

EP - 133

JO - Lait

JF - Lait

SN - 0023-7302

IS - 1-2

ER -