Abstract
The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.
Original language | English |
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Pages (from-to) | 243-250 |
Journal | Biochimica et biophysica acta-protein structure and molecular enzymology |
Volume | 1279 |
DOIs | |
Publication status | Published - 1996 |
Keywords
- Caco-2
- Cholesterol
- Erythrocyte
- Glycoalkaloid
- Membrane disruption
- Mitochondrion
- Permeabilization