Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.

E.A.J. Keukens; T. de Vrije; L.A.M. Jansen; H. de Boer; M. Janssen; A.I.P.M. de Kroon; W.M.F. Jongen; B. de Kruijff

doi:10.1016/0005-2736(95)00253-7

Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.

E.A.J. Keukens, T. de Vrije, L.A.M. Jansen, H. de Boer, M. Janssen, A.I.P.M. de Kroon, W.M.F. Jongen, B. de Kruijff

Research output: Contribution to journal › Article › Academic › peer-review

72 Citations (Scopus)

Abstract

The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.

Original language	English
Pages (from-to)	243-250
Journal	Biochimica et biophysica acta-protein structure and molecular enzymology
Volume	1279
DOIs	https://doi.org/10.1016/0005-2736(95)00253-7
Publication status	Published - 1996

Access to Document

10.1016/0005-2736(95)00253-7Licence: Publisher

Fingerprint Dive into the research topics of 'Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.'. Together they form a unique fingerprint.

Cite this

@article{c303feee4eb343099f00e88447b7470d,

title = "Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.",

abstract = "The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.",

author = "E.A.J. Keukens and {de Vrije}, T. and L.A.M. Jansen and {de Boer}, H. and M. Janssen and {de Kroon}, A.I.P.M. and W.M.F. Jongen and {de Kruijff}, B.",

year = "1996",

doi = "10.1016/0005-2736(95)00253-7",

language = "English",

volume = "1279",

pages = "243--250",

journal = "Biochimica et biophysica acta-protein structure and molecular enzymology",

issn = "0167-4838",

publisher = "Elsevier BV",

}

Glycoalkaloids selectively permeabilize cholesterol containing biomembranes. / Keukens, E.A.J.; de Vrije, T.; Jansen, L.A.M.; de Boer, H.; Janssen, M.; de Kroon, A.I.P.M.; Jongen, W.M.F.; de Kruijff, B.

In: Biochimica et biophysica acta-protein structure and molecular enzymology, Vol. 1279, 1996, p. 243-250.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.

AU - Keukens, E.A.J.

AU - de Vrije, T.

AU - Jansen, L.A.M.

AU - de Boer, H.

AU - Janssen, M.

AU - de Kroon, A.I.P.M.

AU - Jongen, W.M.F.

AU - de Kruijff, B.

PY - 1996

Y1 - 1996

N2 - The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.

AB - The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.

U2 - 10.1016/0005-2736(95)00253-7

DO - 10.1016/0005-2736(95)00253-7

M3 - Article

VL - 1279

SP - 243

EP - 250

JO - Biochimica et biophysica acta-protein structure and molecular enzymology

JF - Biochimica et biophysica acta-protein structure and molecular enzymology

SN - 0167-4838

ER -