Glutathione S-transferase phenotypes in relation to genetic variation and fruit and vegetable consumption in an endoscopy-based population

M.J. Tijhuis, M.H.P.W. Visker, J.M.M.J.G. Aarts, W.H.M. Peters, H.M.J. Roelofs, E.B.G. op den Camp, I.M.C.M. Rietjens, A.M.J.F. Boerboom, F.M. Nagengast, F.J. Kok, E. Kampman

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

High glutathione S-transferase (GST) activity may contribute to colorectal cancer prevention. Functional polymorphisms are known in the GSTM1, GSTT1, GSTA1 and GSTP1 genes. The influence of these GST polymorphisms and recent fruit and vegetable consumption on GST levels and activity has not been investigated simultaneously in a human population. Also, it is not clear if blood GST activity reflects rectal GST activity. Therefore, we determined GST polymorphisms in 94 patients scheduled for sigmoidoscopy. Rectal GST isoenzyme levels (GSTM1, GSTM2, GSTT1, GSTA and GSTP1) were measured by quantitative Western blotting, and rectal and white blood cell total GST activities were measured spectrophotometrically using 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate. Vegetable and fruit consumption was assessed by dietary record. As expected, the GSTM1 and GSTT1 deletion polymorphisms, and the GSTA1 g.-69C>T polymorphism significantly affected the respective isoenzyme levels. Also, rectal GST isoenzyme levels differed between those with and without recent consumption of Alliaceae, Cucurbitaceae, Apiaceae and citrus fruit. Rectal GST activity, however, was not clearly influenced by fruit and vegetable consumption. It was most significantly determined by the GSTP1 c.313A>G polymorphism; compared to the 313AA genotypes, the 313AG and 313GG genotypes showed 36 and 67 nmol/min.mg protein (p
Original languageEnglish
Pages (from-to)848-857
JournalCarcinogenesis
Volume28
Issue number4
DOIs
Publication statusPublished - 2007

Keywords

  • brussels-sprouts
  • aldehyde dehydrogenase
  • cruciferous vegetables
  • colorectal-cancer
  • colon tissue
  • polymorphisms
  • expression
  • alpha
  • risk
  • pi

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