Allotriploid lilies can produce aneuploid or euploid functional female gametes and can be used as the maternal parents in lily introgression breeding. In this study, the genome composition of 14 allopolyploid progenies, including 5 progenies which were derived from LLO×TTTT, 5 progenies which were derived from LLO×OTOT, and 4 BC2 progenies which were derived from MAD×AA/AAAA, were analyzed using genomic in situ hybridization (GISH) technique. The GISH results indicated that all of the 10 progenies which were derived from LLO×TTTT/OTOT were aneuploids, 4 BC2 progenies were euploids. Moreover, LLO always produced aneuploid functional female gametes, giving rise to aneuploid progenies, and MAD produced euploid functional female gametes including n or 2n gametes. Most interestingly, T/O recombinant chromosomes, which were caused by abnormal meiosis, were observed in 2 progenies of LLO×OTOT. Based on the analysis, we proposed that aneuploids might provide a promising approach for lily breeding.
- Genome composition
- Genomic in situ hybridization (GISH)