TY - JOUR
T1 - GISH analyzed progenies generated from allotriploid lilies as female parent
AU - Xi, Mengli
AU - van Tuyl, J.M.
AU - Arens, Paul
PY - 2015
Y1 - 2015
N2 - Allotriploid lilies can produce aneuploid or euploid functional female gametes and can be used as the maternal parents in lily introgression breeding. In this study, the genome composition of 14 allopolyploid progenies, including 5 progenies which were derived from LLO×TTTT, 5 progenies which were derived from LLO×OTOT, and 4 BC2 progenies which were derived from MAD×AA/AAAA, were analyzed using genomic in situ hybridization (GISH) technique. The GISH results indicated that all of the 10 progenies which were derived from LLO×TTTT/OTOT were aneuploids, 4 BC2 progenies were euploids. Moreover, LLO always produced aneuploid functional female gametes, giving rise to aneuploid progenies, and MAD produced euploid functional female gametes including n or 2n gametes. Most interestingly, T/O recombinant chromosomes, which were caused by abnormal meiosis, were observed in 2 progenies of LLO×OTOT. Based on the analysis, we proposed that aneuploids might provide a promising approach for lily breeding.
AB - Allotriploid lilies can produce aneuploid or euploid functional female gametes and can be used as the maternal parents in lily introgression breeding. In this study, the genome composition of 14 allopolyploid progenies, including 5 progenies which were derived from LLO×TTTT, 5 progenies which were derived from LLO×OTOT, and 4 BC2 progenies which were derived from MAD×AA/AAAA, were analyzed using genomic in situ hybridization (GISH) technique. The GISH results indicated that all of the 10 progenies which were derived from LLO×TTTT/OTOT were aneuploids, 4 BC2 progenies were euploids. Moreover, LLO always produced aneuploid functional female gametes, giving rise to aneuploid progenies, and MAD produced euploid functional female gametes including n or 2n gametes. Most interestingly, T/O recombinant chromosomes, which were caused by abnormal meiosis, were observed in 2 progenies of LLO×OTOT. Based on the analysis, we proposed that aneuploids might provide a promising approach for lily breeding.
KW - Aneuploid
KW - Genome composition
KW - Genomic in situ hybridization (GISH)
KW - Lilium
U2 - 10.1016/j.scienta.2014.12.022
DO - 10.1016/j.scienta.2014.12.022
M3 - Article
AN - SCOPUS:84920191803
SN - 0304-4238
VL - 183
SP - 130
EP - 135
JO - Scientia Horticulturae
JF - Scientia Horticulturae
ER -