GISH Analysis of Subsequent Progeny Crossed with 2n-gametes of F-1 Oriental-Asiatic Interspecific Hybrid in Lily

This study was carried out to analyze the chromosome composition and homoeologous recombination of subsequent progenies, derived from cross of Oriental-Asiatic (OA) interspecific hybrid producing 2n-gametes, by GISH technique. GISH analysis of F-1 OA interspecific hybrid '2nOA-001', which produced 2n-gametes, showed 24 chromosomes consisting of 12 chromosomes from Oriental hybrid and 12 chromosomes from Asiatic hybrid. The subsequent backcross progeny of A x OA (2x-2x) crosses produced diploid plants with all Asiatic chromosomes (2x=24) and some triploids (O=12 and A=24), and one aneuploid 2n=35 (O=11 and A=24). Seven plants were produced in the line of 'AOA-006', among them, it was confirmed that three plants were homoeologous recombination with I or 2 breakpoints at short- or long-arm randomly. In case of 0 x OA (2x-2x) crosses, all plants showed 2n=24 chromosomes that originated from Oriental hybrid and no homoeologous recombination. In case of OA x A (2x-2x) crosses, triploid plants (O=12, A=24) were formed without any homoeologous recombination. All progenies were tetraploid plants in the cross of OA x 00 (2x-4x) showing 36 chromosomes that originated from Oriental hybrid and 12 chromosomes originated from Asiatic hybrid, without any homoeologous recombinations.