Germination of Bacillus cereus spores is induced by germinants from differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine

L.M. Wijnands, J.B. Dufrenne, F.M. van Leusden, T. Abee

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.
Original languageEnglish
Pages (from-to)5052-5054
JournalApplied and Environmental Microbiology
Volume73
Issue number15
DOIs
Publication statusPublished - 2007

Fingerprint

Bacillus cereus
Caco-2 Cells
human cell lines
Germination
Spores
Small Intestine
small intestine
spore
germination
epithelial cells
spores
Epithelial Cells
Cell Line
toxigenic strains
spore germination
heart
cells
brain
Food
food

Keywords

  • l-alanine
  • inosine
  • atcc-14579
  • adhesion

Cite this

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abstract = "Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.",
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Germination of Bacillus cereus spores is induced by germinants from differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine. / Wijnands, L.M.; Dufrenne, J.B.; van Leusden, F.M.; Abee, T.

In: Applied and Environmental Microbiology, Vol. 73, No. 15, 2007, p. 5052-5054.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Germination of Bacillus cereus spores is induced by germinants from differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine

AU - Wijnands, L.M.

AU - Dufrenne, J.B.

AU - van Leusden, F.M.

AU - Abee, T.

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PY - 2007

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AB - Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.

KW - l-alanine

KW - inosine

KW - atcc-14579

KW - adhesion

U2 - 10.1128/AEM.02390-06

DO - 10.1128/AEM.02390-06

M3 - Article

VL - 73

SP - 5052

EP - 5054

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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