Germination of Bacillus cereus spores adhered to stainless steel

L.M. Hornstra, P.P.L.A. de Leeuw, R. Moezelaar, E.J.H. Wolbert, Y.P. de Vries, W.M. de Vos, T. Abee

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

Adhered spores of Bacillus cereus represent a significant part of the surface-derived contamination in processing equipment used in the dairy industry. As germinated spores lose their resistance capacities instantaneously, efficient germination prior to a cleaning in place treatment could aid to the disinfecting effect of such a treatment. Therefore, spores of B. cereus ATCC 14579 and that of the environmental isolate B. cereus CMCC 3328 were assessed for their germination behaviour when adhered to a stainless steel surface. A mixture of l-alanine and inosine initiated germination of adhered spores efficiently, resulting in 3.2 decimal logarithms of germination. Notably, implementation of a germination-inducing step prior to a representative cleaning in place procedure reduced the number of survivors with over 3 decimal log units, while an alkali treatment alone, as part of the cleaning in place procedure, did not show any effect on B. cereus spore viability. These results show that implementation of a germination step enhances the disinfection effect of currently used cleaning in place procedures.
Original languageEnglish
Pages (from-to)367-371
JournalInternational Journal of Food Microbiology
Volume116
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • l-alanine
  • surface
  • hydrophobicity
  • removal
  • milk
  • inactivation
  • sporulation
  • resistance
  • sanitizer
  • adhesion

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