Genotyping of pedigreed apple breeding material with a genome covering set of SSRs: Trueness to type of cultivars and their parentages

K.M. Evans, A. Patocchi, F. Rezzonico, F. Mathis-Jeanneteau, C.E. Durel, F. Fernandez-Fernandez, A. Boudichevskaia, F. Dunemann, M. Stankiewicz-Kosyl, L. Gianfranceschi, M. Komjanc, M. Lateur, M. Madduri, Y. Noordijk, W.E. van de Weg

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Apple cultivars and breeding lines that represent much of the diversity currently present in major European breeding programmes and are genetically related by their pedigree were examined for the trueness of their identity and parentage by consistency in marker scores using a genome-covering set of 80 microsatellite (SSR) markers and an ‘identity-by-descent’ approach. One hundred and twenty-five individuals were validated for the trueness-to-type of both their parents and 49 were validated for one of their parents, their second being unknown (23 individuals) or not available in this study (26 individuals). In addition, 15 individuals for which we lacked one of or both the direct parents were validated by consistency with tested parents of earlier generations. Furthermore, the identity of 28 founder cultivars was validated, their marker scores being consistent with descending cultivars and breeding lines. Four of the eight triploids identified were clearly shown to have arisen from unreduced egg cells. The assumed pedigree of 15 further individuals was found to be incorrect; fully consistent pedigrees were suggested for three of the cultivars. The pedigrees of a further eight individuals were confirmed through inference from the molecular data
Original languageEnglish
Pages (from-to)535-547
JournalMolecular Breeding
Volume28
Issue number4
DOIs
Publication statusPublished - 2011

Keywords

  • plant-populations
  • resistance
  • traits

Fingerprint Dive into the research topics of 'Genotyping of pedigreed apple breeding material with a genome covering set of SSRs: Trueness to type of cultivars and their parentages'. Together they form a unique fingerprint.

  • Cite this

    Evans, K. M., Patocchi, A., Rezzonico, F., Mathis-Jeanneteau, F., Durel, C. E., Fernandez-Fernandez, F., Boudichevskaia, A., Dunemann, F., Stankiewicz-Kosyl, M., Gianfranceschi, L., Komjanc, M., Lateur, M., Madduri, M., Noordijk, Y., & van de Weg, W. E. (2011). Genotyping of pedigreed apple breeding material with a genome covering set of SSRs: Trueness to type of cultivars and their parentages. Molecular Breeding, 28(4), 535-547. https://doi.org/10.1007/s11032-010-9502-5