Genotypic differences in metabolomic changes during storage induced-degreening of chrysanthemum disk florets

Geert van Geest*, Young Hae Choi, Paul Arens, Aike Post, Ying Liu, Uulke van Meeteren

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Selecting chrysanthemum cultivars with long storability and vase life is a major challenge for breeders. The rate of degreening of disk florets during the postharvest phase is an important determinant of vase life. There is large genotypic variation in susceptibility to disk floret degreening. Our aim was to understand these genotypic differences at the physiological level. Carbohydrate starvation seemed to play a role, since application of sugars prevented degreening and degreening only occurred if florets had a long-term low carbohydrate content. In order to find out which metabolic processes could explain genotypic differences, we used 1H Nuclear Magnetic Resonance (NMR) spectroscopy profiling, High Performance Anion Exchange Chromatography (HPAEC) and respiration measurements to compare metabolic responses of three genotypes to carbohydrate starvation. HPAEC and NMR measurements showed that carbohydrate content could not fully explain genotypic differences. A genotype with intermediate sensitivity to degreening showed similar carbohydrate content compared to an insensitive one. However, respiration rate declined faster under carbohydrate starvation in a sensitive and intermediate sensitive genotype compared to an insensitive genotype, suggesting a more abrupt constraint on the mitochondrial electron transport chain and with that oxidative stress. Changes in the metabolic profile under carbohydrate starvation were diverse and revealed candidate processes associated with disk floret degreening. Camphor content increased and correlated positively with degreening insensitivity. Phenylpropanoids and flavonoids also increased upon carbohydrate starvation and the response was genotype specific. We propose the upregulation of the phenylpropanoid metabolism as important source of nitrogen in the form of harmful ammonia during carbohydrate starvation. Our results provide a framework to identify processes associated with genotypic differences in the response to carbohydrate starvation and susceptibility to floret degreening.

Original languageEnglish
Pages (from-to)48-59
JournalPostharvest Biology and Technology
Volume115
DOIs
Publication statusPublished - 2016

Fingerprint

degreening
Chrysanthemum
Metabolomics
metabolomics
florets
starvation
Carbohydrates
Starvation
carbohydrates
carbohydrate content
Genotype
genotype
vase life
phenylpropanoids
nuclear magnetic resonance spectroscopy
Anions
Chromatography
Magnetic Resonance Spectroscopy
camphor
electron transport chain

Keywords

  • Carbohydrate starvation
  • Chrysanthemum
  • Metabolomics
  • Respiration
  • Senescence
  • Storage

Cite this

@article{5e8c4796d40f4480b8faf6393388cb3d,
title = "Genotypic differences in metabolomic changes during storage induced-degreening of chrysanthemum disk florets",
abstract = "Selecting chrysanthemum cultivars with long storability and vase life is a major challenge for breeders. The rate of degreening of disk florets during the postharvest phase is an important determinant of vase life. There is large genotypic variation in susceptibility to disk floret degreening. Our aim was to understand these genotypic differences at the physiological level. Carbohydrate starvation seemed to play a role, since application of sugars prevented degreening and degreening only occurred if florets had a long-term low carbohydrate content. In order to find out which metabolic processes could explain genotypic differences, we used 1H Nuclear Magnetic Resonance (NMR) spectroscopy profiling, High Performance Anion Exchange Chromatography (HPAEC) and respiration measurements to compare metabolic responses of three genotypes to carbohydrate starvation. HPAEC and NMR measurements showed that carbohydrate content could not fully explain genotypic differences. A genotype with intermediate sensitivity to degreening showed similar carbohydrate content compared to an insensitive one. However, respiration rate declined faster under carbohydrate starvation in a sensitive and intermediate sensitive genotype compared to an insensitive genotype, suggesting a more abrupt constraint on the mitochondrial electron transport chain and with that oxidative stress. Changes in the metabolic profile under carbohydrate starvation were diverse and revealed candidate processes associated with disk floret degreening. Camphor content increased and correlated positively with degreening insensitivity. Phenylpropanoids and flavonoids also increased upon carbohydrate starvation and the response was genotype specific. We propose the upregulation of the phenylpropanoid metabolism as important source of nitrogen in the form of harmful ammonia during carbohydrate starvation. Our results provide a framework to identify processes associated with genotypic differences in the response to carbohydrate starvation and susceptibility to floret degreening.",
keywords = "Carbohydrate starvation, Chrysanthemum, Metabolomics, Respiration, Senescence, Storage",
author = "{van Geest}, Geert and Choi, {Young Hae} and Paul Arens and Aike Post and Ying Liu and {van Meeteren}, Uulke",
year = "2016",
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journal = "Postharvest Biology and Technology",
issn = "0925-5214",
publisher = "Elsevier",

}

Genotypic differences in metabolomic changes during storage induced-degreening of chrysanthemum disk florets. / van Geest, Geert; Choi, Young Hae; Arens, Paul; Post, Aike; Liu, Ying; van Meeteren, Uulke.

In: Postharvest Biology and Technology, Vol. 115, 2016, p. 48-59.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genotypic differences in metabolomic changes during storage induced-degreening of chrysanthemum disk florets

AU - van Geest, Geert

AU - Choi, Young Hae

AU - Arens, Paul

AU - Post, Aike

AU - Liu, Ying

AU - van Meeteren, Uulke

PY - 2016

Y1 - 2016

N2 - Selecting chrysanthemum cultivars with long storability and vase life is a major challenge for breeders. The rate of degreening of disk florets during the postharvest phase is an important determinant of vase life. There is large genotypic variation in susceptibility to disk floret degreening. Our aim was to understand these genotypic differences at the physiological level. Carbohydrate starvation seemed to play a role, since application of sugars prevented degreening and degreening only occurred if florets had a long-term low carbohydrate content. In order to find out which metabolic processes could explain genotypic differences, we used 1H Nuclear Magnetic Resonance (NMR) spectroscopy profiling, High Performance Anion Exchange Chromatography (HPAEC) and respiration measurements to compare metabolic responses of three genotypes to carbohydrate starvation. HPAEC and NMR measurements showed that carbohydrate content could not fully explain genotypic differences. A genotype with intermediate sensitivity to degreening showed similar carbohydrate content compared to an insensitive one. However, respiration rate declined faster under carbohydrate starvation in a sensitive and intermediate sensitive genotype compared to an insensitive genotype, suggesting a more abrupt constraint on the mitochondrial electron transport chain and with that oxidative stress. Changes in the metabolic profile under carbohydrate starvation were diverse and revealed candidate processes associated with disk floret degreening. Camphor content increased and correlated positively with degreening insensitivity. Phenylpropanoids and flavonoids also increased upon carbohydrate starvation and the response was genotype specific. We propose the upregulation of the phenylpropanoid metabolism as important source of nitrogen in the form of harmful ammonia during carbohydrate starvation. Our results provide a framework to identify processes associated with genotypic differences in the response to carbohydrate starvation and susceptibility to floret degreening.

AB - Selecting chrysanthemum cultivars with long storability and vase life is a major challenge for breeders. The rate of degreening of disk florets during the postharvest phase is an important determinant of vase life. There is large genotypic variation in susceptibility to disk floret degreening. Our aim was to understand these genotypic differences at the physiological level. Carbohydrate starvation seemed to play a role, since application of sugars prevented degreening and degreening only occurred if florets had a long-term low carbohydrate content. In order to find out which metabolic processes could explain genotypic differences, we used 1H Nuclear Magnetic Resonance (NMR) spectroscopy profiling, High Performance Anion Exchange Chromatography (HPAEC) and respiration measurements to compare metabolic responses of three genotypes to carbohydrate starvation. HPAEC and NMR measurements showed that carbohydrate content could not fully explain genotypic differences. A genotype with intermediate sensitivity to degreening showed similar carbohydrate content compared to an insensitive one. However, respiration rate declined faster under carbohydrate starvation in a sensitive and intermediate sensitive genotype compared to an insensitive genotype, suggesting a more abrupt constraint on the mitochondrial electron transport chain and with that oxidative stress. Changes in the metabolic profile under carbohydrate starvation were diverse and revealed candidate processes associated with disk floret degreening. Camphor content increased and correlated positively with degreening insensitivity. Phenylpropanoids and flavonoids also increased upon carbohydrate starvation and the response was genotype specific. We propose the upregulation of the phenylpropanoid metabolism as important source of nitrogen in the form of harmful ammonia during carbohydrate starvation. Our results provide a framework to identify processes associated with genotypic differences in the response to carbohydrate starvation and susceptibility to floret degreening.

KW - Carbohydrate starvation

KW - Chrysanthemum

KW - Metabolomics

KW - Respiration

KW - Senescence

KW - Storage

U2 - 10.1016/j.postharvbio.2015.12.008

DO - 10.1016/j.postharvbio.2015.12.008

M3 - Article

VL - 115

SP - 48

EP - 59

JO - Postharvest Biology and Technology

JF - Postharvest Biology and Technology

SN - 0925-5214

ER -