Genomics of tailless bacteriophages in a complex lactic acid bacteria starter culture

Svetlana Alexeeva; Yue Liu; Jingjie Zhu; Joanna Kaczorowska; Thijs R.H.M. Kouwen; Tjakko Abee; Eddy J. Smid

doi:10.1016/j.idairyj.2020.104900

Genomics of tailless bacteriophages in a complex lactic acid bacteria starter culture

Svetlana Alexeeva, Yue Liu, Jingjie Zhu, Joanna Kaczorowska, Thijs R.H.M. Kouwen, Tjakko Abee, Eddy J. Smid^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

Our previous study on a model microbial community originating from an artisanal cheese fermentation starter revealed that bacteriophages not only co-exist with bacteria but also are highly abundant. Here we describe the genomic content of phage particles released by 6 different strains in the starter culture. The identified prophages belong to three different subgroups of the Siphoviridae P335 phage group. Remarkably, most analysed prophages show disruptions in different tail encoding genes, explaining the common tailless phenotype. Furthermore, a number of potentially beneficial features for the host carried by prophages were identified. The prophages carry up to 3 different phage defence systems per genome that are potentially functional in protecting the host from foreign phage infection. We suggest that the presumably defective prophages are a result of bacteria-phage coevolution and could convey advantages to host bacteria.

Original language	English
Article number	104900
Journal	International Dairy Journal
Volume	114
DOIs	https://doi.org/10.1016/j.idairyj.2020.104900
Publication status	Published - Mar 2021

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title = "Genomics of tailless bacteriophages in a complex lactic acid bacteria starter culture",

abstract = "Our previous study on a model microbial community originating from an artisanal cheese fermentation starter revealed that bacteriophages not only co-exist with bacteria but also are highly abundant. Here we describe the genomic content of phage particles released by 6 different strains in the starter culture. The identified prophages belong to three different subgroups of the Siphoviridae P335 phage group. Remarkably, most analysed prophages show disruptions in different tail encoding genes, explaining the common tailless phenotype. Furthermore, a number of potentially beneficial features for the host carried by prophages were identified. The prophages carry up to 3 different phage defence systems per genome that are potentially functional in protecting the host from foreign phage infection. We suggest that the presumably defective prophages are a result of bacteria-phage coevolution and could convey advantages to host bacteria.",

author = "Svetlana Alexeeva and Yue Liu and Jingjie Zhu and Joanna Kaczorowska and Kouwen, {Thijs R.H.M.} and Tjakko Abee and Smid, {Eddy J.}",

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doi = "10.1016/j.idairyj.2020.104900",

language = "English",

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journal = "International Dairy Journal",

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T1 - Genomics of tailless bacteriophages in a complex lactic acid bacteria starter culture

AU - Alexeeva, Svetlana

AU - Liu, Yue

AU - Zhu, Jingjie

AU - Kaczorowska, Joanna

AU - Kouwen, Thijs R.H.M.

AU - Abee, Tjakko

AU - Smid, Eddy J.

PY - 2021/3

Y1 - 2021/3

N2 - Our previous study on a model microbial community originating from an artisanal cheese fermentation starter revealed that bacteriophages not only co-exist with bacteria but also are highly abundant. Here we describe the genomic content of phage particles released by 6 different strains in the starter culture. The identified prophages belong to three different subgroups of the Siphoviridae P335 phage group. Remarkably, most analysed prophages show disruptions in different tail encoding genes, explaining the common tailless phenotype. Furthermore, a number of potentially beneficial features for the host carried by prophages were identified. The prophages carry up to 3 different phage defence systems per genome that are potentially functional in protecting the host from foreign phage infection. We suggest that the presumably defective prophages are a result of bacteria-phage coevolution and could convey advantages to host bacteria.

AB - Our previous study on a model microbial community originating from an artisanal cheese fermentation starter revealed that bacteriophages not only co-exist with bacteria but also are highly abundant. Here we describe the genomic content of phage particles released by 6 different strains in the starter culture. The identified prophages belong to three different subgroups of the Siphoviridae P335 phage group. Remarkably, most analysed prophages show disruptions in different tail encoding genes, explaining the common tailless phenotype. Furthermore, a number of potentially beneficial features for the host carried by prophages were identified. The prophages carry up to 3 different phage defence systems per genome that are potentially functional in protecting the host from foreign phage infection. We suggest that the presumably defective prophages are a result of bacteria-phage coevolution and could convey advantages to host bacteria.

U2 - 10.1016/j.idairyj.2020.104900

DO - 10.1016/j.idairyj.2020.104900

M3 - Article

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SN - 0958-6946

VL - 114

JO - International Dairy Journal

JF - International Dairy Journal

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ER -

Genomics of tailless bacteriophages in a complex lactic acid bacteria starter culture

Abstract

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