Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds

Liesbeth François, Hanne Hoskens, Brandon D. Velie, Anneleen Stinckens, Susanne Tinel, Chris Lamberigts, Liesbet Peeters, Huub F.J. Savelkoul, Edwin Tijhaar, Gabriella Lindgren, Steven Janssens, Bart J. Ducro, Nadine Buys, Anouk Schurink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Insect bite hypersensitivity (IBH), which is a cutaneous allergic reaction to antigens from Culicoides spp., is the most prevalent skin disorder in horses. Misdiagnosis is possible, as IBH is usually diagnosed based on clinical signs. Our study is the first to employ IgE levels against several recombinant Culicoides spp. allergens as an objective, independent, and quantitative phenotype to improve the power to detect genetic variants that underlie IBH. Genotypes of 200 Shetland ponies, 127 Icelandic horses, and 223 Belgian Warmblood horses were analyzed while using a mixed model approach. No single-nucleotide polymorphism (SNP) passed the Bonferroni corrected significance threshold, but several regions were identified within and across breeds, which confirmed previously identified regions of interest and, in addition, identifying new regions of interest. Allergen-specific IgE levels are a continuous and objective phenotype that allow for more powerful analyses when compared to a case-control set-up, as more significant associations were obtained. However, the use of a higher density array seems necessary to fully employ the use of IgE levels as a phenotype. While these results still require validation in a large independent dataset, the use of allergen-specific IgE levels showed value as an objective and continuous phenotype that can deepen our understanding of the biology underlying IBH.

Original languageEnglish
Article number597
JournalGenes
Volume10
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

Fingerprint

Ceratopogonidae
Insect Bites and Stings
Immunoglobulin E
Horses
Hypersensitivity
Antigens
Allergens
Phenotype
Skin
Diagnostic Errors
Single Nucleotide Polymorphism
Genotype

Keywords

  • Belgian Warmblood horse
  • Diagnostic ELISA test
  • Genome-wide association study
  • Icelandic horse
  • IgE
  • Insect bite hypersensitivity
  • Shetland pony
  • Summer eczema

Cite this

François, L., Hoskens, H., Velie, B. D., Stinckens, A., Tinel, S., Lamberigts, C., ... Schurink, A. (2019). Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds. Genes, 10(8), [597]. https://doi.org/10.3390/genes10080597
François, Liesbeth ; Hoskens, Hanne ; Velie, Brandon D. ; Stinckens, Anneleen ; Tinel, Susanne ; Lamberigts, Chris ; Peeters, Liesbet ; Savelkoul, Huub F.J. ; Tijhaar, Edwin ; Lindgren, Gabriella ; Janssens, Steven ; Ducro, Bart J. ; Buys, Nadine ; Schurink, Anouk. / Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds. In: Genes. 2019 ; Vol. 10, No. 8.
@article{122e05d5874f4ae4a13ba40698dafc8f,
title = "Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds",
abstract = "Insect bite hypersensitivity (IBH), which is a cutaneous allergic reaction to antigens from Culicoides spp., is the most prevalent skin disorder in horses. Misdiagnosis is possible, as IBH is usually diagnosed based on clinical signs. Our study is the first to employ IgE levels against several recombinant Culicoides spp. allergens as an objective, independent, and quantitative phenotype to improve the power to detect genetic variants that underlie IBH. Genotypes of 200 Shetland ponies, 127 Icelandic horses, and 223 Belgian Warmblood horses were analyzed while using a mixed model approach. No single-nucleotide polymorphism (SNP) passed the Bonferroni corrected significance threshold, but several regions were identified within and across breeds, which confirmed previously identified regions of interest and, in addition, identifying new regions of interest. Allergen-specific IgE levels are a continuous and objective phenotype that allow for more powerful analyses when compared to a case-control set-up, as more significant associations were obtained. However, the use of a higher density array seems necessary to fully employ the use of IgE levels as a phenotype. While these results still require validation in a large independent dataset, the use of allergen-specific IgE levels showed value as an objective and continuous phenotype that can deepen our understanding of the biology underlying IBH.",
keywords = "Belgian Warmblood horse, Diagnostic ELISA test, Genome-wide association study, Icelandic horse, IgE, Insect bite hypersensitivity, Shetland pony, Summer eczema",
author = "Liesbeth Fran{\cc}ois and Hanne Hoskens and Velie, {Brandon D.} and Anneleen Stinckens and Susanne Tinel and Chris Lamberigts and Liesbet Peeters and Savelkoul, {Huub F.J.} and Edwin Tijhaar and Gabriella Lindgren and Steven Janssens and Ducro, {Bart J.} and Nadine Buys and Anouk Schurink",
year = "2019",
month = "8",
day = "1",
doi = "10.3390/genes10080597",
language = "English",
volume = "10",
journal = "Genes",
issn = "2073-4425",
publisher = "MDPI",
number = "8",

}

François, L, Hoskens, H, Velie, BD, Stinckens, A, Tinel, S, Lamberigts, C, Peeters, L, Savelkoul, HFJ, Tijhaar, E, Lindgren, G, Janssens, S, Ducro, BJ, Buys, N & Schurink, A 2019, 'Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds', Genes, vol. 10, no. 8, 597. https://doi.org/10.3390/genes10080597

Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds. / François, Liesbeth; Hoskens, Hanne; Velie, Brandon D.; Stinckens, Anneleen; Tinel, Susanne; Lamberigts, Chris; Peeters, Liesbet; Savelkoul, Huub F.J.; Tijhaar, Edwin; Lindgren, Gabriella; Janssens, Steven; Ducro, Bart J.; Buys, Nadine; Schurink, Anouk.

In: Genes, Vol. 10, No. 8, 597, 01.08.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds

AU - François, Liesbeth

AU - Hoskens, Hanne

AU - Velie, Brandon D.

AU - Stinckens, Anneleen

AU - Tinel, Susanne

AU - Lamberigts, Chris

AU - Peeters, Liesbet

AU - Savelkoul, Huub F.J.

AU - Tijhaar, Edwin

AU - Lindgren, Gabriella

AU - Janssens, Steven

AU - Ducro, Bart J.

AU - Buys, Nadine

AU - Schurink, Anouk

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Insect bite hypersensitivity (IBH), which is a cutaneous allergic reaction to antigens from Culicoides spp., is the most prevalent skin disorder in horses. Misdiagnosis is possible, as IBH is usually diagnosed based on clinical signs. Our study is the first to employ IgE levels against several recombinant Culicoides spp. allergens as an objective, independent, and quantitative phenotype to improve the power to detect genetic variants that underlie IBH. Genotypes of 200 Shetland ponies, 127 Icelandic horses, and 223 Belgian Warmblood horses were analyzed while using a mixed model approach. No single-nucleotide polymorphism (SNP) passed the Bonferroni corrected significance threshold, but several regions were identified within and across breeds, which confirmed previously identified regions of interest and, in addition, identifying new regions of interest. Allergen-specific IgE levels are a continuous and objective phenotype that allow for more powerful analyses when compared to a case-control set-up, as more significant associations were obtained. However, the use of a higher density array seems necessary to fully employ the use of IgE levels as a phenotype. While these results still require validation in a large independent dataset, the use of allergen-specific IgE levels showed value as an objective and continuous phenotype that can deepen our understanding of the biology underlying IBH.

AB - Insect bite hypersensitivity (IBH), which is a cutaneous allergic reaction to antigens from Culicoides spp., is the most prevalent skin disorder in horses. Misdiagnosis is possible, as IBH is usually diagnosed based on clinical signs. Our study is the first to employ IgE levels against several recombinant Culicoides spp. allergens as an objective, independent, and quantitative phenotype to improve the power to detect genetic variants that underlie IBH. Genotypes of 200 Shetland ponies, 127 Icelandic horses, and 223 Belgian Warmblood horses were analyzed while using a mixed model approach. No single-nucleotide polymorphism (SNP) passed the Bonferroni corrected significance threshold, but several regions were identified within and across breeds, which confirmed previously identified regions of interest and, in addition, identifying new regions of interest. Allergen-specific IgE levels are a continuous and objective phenotype that allow for more powerful analyses when compared to a case-control set-up, as more significant associations were obtained. However, the use of a higher density array seems necessary to fully employ the use of IgE levels as a phenotype. While these results still require validation in a large independent dataset, the use of allergen-specific IgE levels showed value as an objective and continuous phenotype that can deepen our understanding of the biology underlying IBH.

KW - Belgian Warmblood horse

KW - Diagnostic ELISA test

KW - Genome-wide association study

KW - Icelandic horse

KW - IgE

KW - Insect bite hypersensitivity

KW - Shetland pony

KW - Summer eczema

U2 - 10.3390/genes10080597

DO - 10.3390/genes10080597

M3 - Article

VL - 10

JO - Genes

JF - Genes

SN - 2073-4425

IS - 8

M1 - 597

ER -

François L, Hoskens H, Velie BD, Stinckens A, Tinel S, Lamberigts C et al. Genomic regions associated with IgE levels against culicoides spp. Antigens in three horse breeds. Genes. 2019 Aug 1;10(8). 597. https://doi.org/10.3390/genes10080597