Genomic rearrangements drive effector evolution in Verticillium dahliae

L. Faino, M.F. Seidl, G.C.M. van den Berg, E. Datema, A. Janssen, A. Wittenberg, B.P.H.J. Thomma

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

P114 - Verticillium dahliae is the causal agent of tomato vascular wilt disease. While race 1 isolates express the Ave1 effector that is recognized by the tomato Ve1 immune receptor, race 2 isolates lacks this effector that was identified based on comparative genomics. Comparative genomics further revealed extensive genomic rearrangements between V. dahliae strains leading to lineage-specific regions involved in virulence and niche adaptation. To understand the role of rearrangements in effector evolution between V. dahliae strains, we re- sequenced the ~37 Mb genome of V. dahliae strain JR2 using PacBio and assembled a gapless genome which was aligned to the assembly of strain Ls17 and synteny breakpoints were identified. Subsequently, we assessed the genomic localizations of in planta-induced genes and we prove that virulence genes localize in lineage- specific regions and these regions are in close proximity to genomic rearrangements. Furthermore, we focused on the well-characterized effector Ave1 which localizes in a region were rearrangements occur. Aligning of Illumina reads of 10 V. dahliae strains to the gapless JR2 genome revealed that the Ave1 locus was lost multiple times during evolution.
Original languageEnglish
Title of host publicationBook of Abstracts XVI International Congress on Molecular Plant-Microbe Interactions
Pages57
Publication statusPublished - 2014
EventXVI International Congress on Molecular Plant-Microbe Interactions, Rhodes, Greece -
Duration: 6 Jul 201410 Jul 2014

Conference

ConferenceXVI International Congress on Molecular Plant-Microbe Interactions, Rhodes, Greece
Period6/07/1410/07/14

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