P114 - Verticillium dahliae is the causal agent of tomato vascular wilt disease. While race 1 isolates express the Ave1 effector that is recognized by the tomato Ve1 immune receptor, race 2 isolates lacks this effector that was identified based on comparative genomics. Comparative genomics further revealed extensive genomic rearrangements between V. dahliae strains leading to lineage-specific regions involved in virulence and niche adaptation. To understand the role of rearrangements in effector evolution between V. dahliae strains, we re- sequenced the ~37 Mb genome of V. dahliae strain JR2 using PacBio and assembled a gapless genome which was aligned to the assembly of strain Ls17 and synteny breakpoints were identified. Subsequently, we assessed the genomic localizations of in planta-induced genes and we prove that virulence genes localize in lineage- specific regions and these regions are in close proximity to genomic rearrangements. Furthermore, we focused on the well-characterized effector Ave1 which localizes in a region were rearrangements occur. Aligning of Illumina reads of 10 V. dahliae strains to the gapless JR2 genome revealed that the Ave1 locus was lost multiple times during evolution.
|Title of host publication||Book of Abstracts XVI International Congress on Molecular Plant-Microbe Interactions|
|Publication status||Published - 2014|
|Event||XVI International Congress on Molecular Plant-Microbe Interactions, Rhodes, Greece - |
Duration: 6 Jul 2014 → 10 Jul 2014
|Conference||XVI International Congress on Molecular Plant-Microbe Interactions, Rhodes, Greece|
|Period||6/07/14 → 10/07/14|