Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin)

Z.S. Gao, W.E. van de Weg, J.G. Schaart, G. van Arkel, H. Breiteneder, K. Hoffmann-Sommergruber, L.J.W.J. Gilissen

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Four classes of apple allergens (Mal d 1, ¿2, ¿3 and ¿4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01A and Mal d 2.01B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01A and Mal d 2.01B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01A and B, Mal d 4.02A and Mal d 4.03A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02A and Mal d 4.03A were single copy genes located on linkage group 2 and 8, respectively
Original languageEnglish
Pages (from-to)1087-1097
JournalTheoretical and Applied Genetics
Volume111
Issue number6
DOIs
Publication statusPublished - 2005

Fingerprint

Profilins
Chromosome Mapping
Malus
allergens
Allergens
chromosome mapping
apples
linkage groups
genomics
Genes
Proteins
genes
proteins
Protein Sorting Signals
signal peptide
Introns
exons
Organism Cloning
introns
molecular cloning

Keywords

  • cross-reactivity
  • malus-domestica
  • pollen profilin
  • heterologous expression
  • cloning
  • arabidopsis
  • family
  • ige
  • identification
  • potency

Cite this

@article{d78cabb3748c4c3b9615ad8f9e2effa2,
title = "Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin)",
abstract = "Four classes of apple allergens (Mal d 1, ¿2, ¿3 and ¿4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01A and Mal d 2.01B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01A and Mal d 2.01B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01A and B, Mal d 4.02A and Mal d 4.03A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02A and Mal d 4.03A were single copy genes located on linkage group 2 and 8, respectively",
keywords = "cross-reactivity, malus-domestica, pollen profilin, heterologous expression, cloning, arabidopsis, family, ige, identification, potency",
author = "Z.S. Gao and {van de Weg}, W.E. and J.G. Schaart and {van Arkel}, G. and H. Breiteneder and K. Hoffmann-Sommergruber and L.J.W.J. Gilissen",
year = "2005",
doi = "10.1007/s00122-005-0034-z",
language = "English",
volume = "111",
pages = "1087--1097",
journal = "Theoretical and Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
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}

Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin). / Gao, Z.S.; van de Weg, W.E.; Schaart, J.G.; van Arkel, G.; Breiteneder, H.; Hoffmann-Sommergruber, K.; Gilissen, L.J.W.J.

In: Theoretical and Applied Genetics, Vol. 111, No. 6, 2005, p. 1087-1097.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin)

AU - Gao, Z.S.

AU - van de Weg, W.E.

AU - Schaart, J.G.

AU - van Arkel, G.

AU - Breiteneder, H.

AU - Hoffmann-Sommergruber, K.

AU - Gilissen, L.J.W.J.

PY - 2005

Y1 - 2005

N2 - Four classes of apple allergens (Mal d 1, ¿2, ¿3 and ¿4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01A and Mal d 2.01B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01A and Mal d 2.01B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01A and B, Mal d 4.02A and Mal d 4.03A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02A and Mal d 4.03A were single copy genes located on linkage group 2 and 8, respectively

AB - Four classes of apple allergens (Mal d 1, ¿2, ¿3 and ¿4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01A and Mal d 2.01B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01A and Mal d 2.01B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01A and B, Mal d 4.02A and Mal d 4.03A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02A and Mal d 4.03A were single copy genes located on linkage group 2 and 8, respectively

KW - cross-reactivity

KW - malus-domestica

KW - pollen profilin

KW - heterologous expression

KW - cloning

KW - arabidopsis

KW - family

KW - ige

KW - identification

KW - potency

U2 - 10.1007/s00122-005-0034-z

DO - 10.1007/s00122-005-0034-z

M3 - Article

VL - 111

SP - 1087

EP - 1097

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 6

ER -