Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin)

Z.S. Gao, W.E. van de Weg, J.G. Schaart, G. van Arkel, H. Breiteneder, K. Hoffmann-Sommergruber, L.J.W.J. Gilissen

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Abstract

Four classes of apple allergens (Mal d 1, ¿2, ¿3 and ¿4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01A and Mal d 2.01B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01A and Mal d 2.01B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01A and B, Mal d 4.02A and Mal d 4.03A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02A and Mal d 4.03A were single copy genes located on linkage group 2 and 8, respectively
Original languageEnglish
Pages (from-to)1087-1097
JournalTheoretical and Applied Genetics
Volume111
Issue number6
DOIs
Publication statusPublished - 2005

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Keywords

  • cross-reactivity
  • malus-domestica
  • pollen profilin
  • heterologous expression
  • cloning
  • arabidopsis
  • family
  • ige
  • identification
  • potency

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