Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats

R.J.M. Volkers, L.B. Snoek, J.A.G. Riksen, H. Schulenburg, B. Braeckman, J.E. Kammenga

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats Rita J Volkers¹, Basten L Snoek¹, Joost Riksen¹, Hinrich Schulenburg², Bart Braeckman³, Jan E Kammenga¹ 1Wageningen University, Nematology, Wageningen, 6708PB, Netherlands, ²Christian-Albrechts-University of Kiel, Evolutionary Ecology and Genetics, Kiel, 24118, Germany, ³Ghent University, Biology, Ghent, B-9000, Belgium Local populations of C. elegans are genetically diverse and low frequency of out-crossing allows for the recombination of these locally diverse genotypes. Environmental challenges may cause a fitness reduction and therefore impose selection on the affected populations. Two main responses are possible. i) A phenotypic plastic response in which selection favored adaptation to environmental factors by adjusting metabolic processes or specific behaviors. Adaptation is thus determined at the non-genetic level (i.e. at the transcriptomic or proteomic level); ii) an evolutionary response in which selection directly acts on the genetic composition of the population as a consequence of differential mortality and reproductive success. Adaptation to the environmental challenge is thus manifested genetically within the population. Yet, we currently lack any understanding concerning adaptive responses in C. elegans to environmental conditions. Here we evaluate the importance of the two adaptive responses and the underlying genomic architecture, using 40 natural populations derived from different habitats and locations in France (kindly provided by M-A Félix). Half of them were isolated from rotting apples in Orsay and the other half from rotting hogweed stems in Santeuil. As an out-group we used 3 newly isolated Dutch strains. We found that populations from the two habitats could be distinctively separated by genotypic differences as well as differential genome-wide gene expression levels and various life-history traits. Currently we investigate the response to locally associated bacteria and the possible genetic adaptations to these different bacteria. Together our results will lead to a more complete understanding of the adaptation and evolution of C. elegans. This work is supported the European Science Foundation (ESF): NEMADAPT - Molecular architecture of environmental adaptation in natural populations of the nematode Caenorhabditis elegans.
Original languageEnglish
Title of host publicationAbstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012
Pages92-92
Publication statusPublished - 2012
EventEvolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA -
Duration: 3 Apr 20126 Apr 2012

Conference

ConferenceEvolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA
Period3/04/126/04/12

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Caenorhabditis elegans
genomics
habitats
selection response
nematology
environmental factors
bacteria
outcrossing
transcriptomics
Belgium
proteomics
Netherlands
plastics
apples
France
Germany
life history
Nematoda
ecology
Biological Sciences

Cite this

Volkers, R. J. M., Snoek, L. B., Riksen, J. A. G., Schulenburg, H., Braeckman, B., & Kammenga, J. E. (2012). Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats. In Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012 (pp. 92-92)
Volkers, R.J.M. ; Snoek, L.B. ; Riksen, J.A.G. ; Schulenburg, H. ; Braeckman, B. ; Kammenga, J.E. / Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats. Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012. 2012. pp. 92-92
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title = "Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats",
abstract = "Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats Rita J Volkers¹, Basten L Snoek¹, Joost Riksen¹, Hinrich Schulenburg², Bart Braeckman³, Jan E Kammenga¹ 1Wageningen University, Nematology, Wageningen, 6708PB, Netherlands, ²Christian-Albrechts-University of Kiel, Evolutionary Ecology and Genetics, Kiel, 24118, Germany, ³Ghent University, Biology, Ghent, B-9000, Belgium Local populations of C. elegans are genetically diverse and low frequency of out-crossing allows for the recombination of these locally diverse genotypes. Environmental challenges may cause a fitness reduction and therefore impose selection on the affected populations. Two main responses are possible. i) A phenotypic plastic response in which selection favored adaptation to environmental factors by adjusting metabolic processes or specific behaviors. Adaptation is thus determined at the non-genetic level (i.e. at the transcriptomic or proteomic level); ii) an evolutionary response in which selection directly acts on the genetic composition of the population as a consequence of differential mortality and reproductive success. Adaptation to the environmental challenge is thus manifested genetically within the population. Yet, we currently lack any understanding concerning adaptive responses in C. elegans to environmental conditions. Here we evaluate the importance of the two adaptive responses and the underlying genomic architecture, using 40 natural populations derived from different habitats and locations in France (kindly provided by M-A F{\'e}lix). Half of them were isolated from rotting apples in Orsay and the other half from rotting hogweed stems in Santeuil. As an out-group we used 3 newly isolated Dutch strains. We found that populations from the two habitats could be distinctively separated by genotypic differences as well as differential genome-wide gene expression levels and various life-history traits. Currently we investigate the response to locally associated bacteria and the possible genetic adaptations to these different bacteria. Together our results will lead to a more complete understanding of the adaptation and evolution of C. elegans. This work is supported the European Science Foundation (ESF): NEMADAPT - Molecular architecture of environmental adaptation in natural populations of the nematode Caenorhabditis elegans.",
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Volkers, RJM, Snoek, LB, Riksen, JAG, Schulenburg, H, Braeckman, B & Kammenga, JE 2012, Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats. in Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012. pp. 92-92, Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3/04/12.

Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats. / Volkers, R.J.M.; Snoek, L.B.; Riksen, J.A.G.; Schulenburg, H.; Braeckman, B.; Kammenga, J.E.

Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012. 2012. p. 92-92.

Research output: Chapter in Book/Report/Conference proceedingAbstract

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T1 - Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats

AU - Volkers, R.J.M.

AU - Snoek, L.B.

AU - Riksen, J.A.G.

AU - Schulenburg, H.

AU - Braeckman, B.

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N2 - Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats Rita J Volkers¹, Basten L Snoek¹, Joost Riksen¹, Hinrich Schulenburg², Bart Braeckman³, Jan E Kammenga¹ 1Wageningen University, Nematology, Wageningen, 6708PB, Netherlands, ²Christian-Albrechts-University of Kiel, Evolutionary Ecology and Genetics, Kiel, 24118, Germany, ³Ghent University, Biology, Ghent, B-9000, Belgium Local populations of C. elegans are genetically diverse and low frequency of out-crossing allows for the recombination of these locally diverse genotypes. Environmental challenges may cause a fitness reduction and therefore impose selection on the affected populations. Two main responses are possible. i) A phenotypic plastic response in which selection favored adaptation to environmental factors by adjusting metabolic processes or specific behaviors. Adaptation is thus determined at the non-genetic level (i.e. at the transcriptomic or proteomic level); ii) an evolutionary response in which selection directly acts on the genetic composition of the population as a consequence of differential mortality and reproductive success. Adaptation to the environmental challenge is thus manifested genetically within the population. Yet, we currently lack any understanding concerning adaptive responses in C. elegans to environmental conditions. Here we evaluate the importance of the two adaptive responses and the underlying genomic architecture, using 40 natural populations derived from different habitats and locations in France (kindly provided by M-A Félix). Half of them were isolated from rotting apples in Orsay and the other half from rotting hogweed stems in Santeuil. As an out-group we used 3 newly isolated Dutch strains. We found that populations from the two habitats could be distinctively separated by genotypic differences as well as differential genome-wide gene expression levels and various life-history traits. Currently we investigate the response to locally associated bacteria and the possible genetic adaptations to these different bacteria. Together our results will lead to a more complete understanding of the adaptation and evolution of C. elegans. This work is supported the European Science Foundation (ESF): NEMADAPT - Molecular architecture of environmental adaptation in natural populations of the nematode Caenorhabditis elegans.

AB - Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats Rita J Volkers¹, Basten L Snoek¹, Joost Riksen¹, Hinrich Schulenburg², Bart Braeckman³, Jan E Kammenga¹ 1Wageningen University, Nematology, Wageningen, 6708PB, Netherlands, ²Christian-Albrechts-University of Kiel, Evolutionary Ecology and Genetics, Kiel, 24118, Germany, ³Ghent University, Biology, Ghent, B-9000, Belgium Local populations of C. elegans are genetically diverse and low frequency of out-crossing allows for the recombination of these locally diverse genotypes. Environmental challenges may cause a fitness reduction and therefore impose selection on the affected populations. Two main responses are possible. i) A phenotypic plastic response in which selection favored adaptation to environmental factors by adjusting metabolic processes or specific behaviors. Adaptation is thus determined at the non-genetic level (i.e. at the transcriptomic or proteomic level); ii) an evolutionary response in which selection directly acts on the genetic composition of the population as a consequence of differential mortality and reproductive success. Adaptation to the environmental challenge is thus manifested genetically within the population. Yet, we currently lack any understanding concerning adaptive responses in C. elegans to environmental conditions. Here we evaluate the importance of the two adaptive responses and the underlying genomic architecture, using 40 natural populations derived from different habitats and locations in France (kindly provided by M-A Félix). Half of them were isolated from rotting apples in Orsay and the other half from rotting hogweed stems in Santeuil. As an out-group we used 3 newly isolated Dutch strains. We found that populations from the two habitats could be distinctively separated by genotypic differences as well as differential genome-wide gene expression levels and various life-history traits. Currently we investigate the response to locally associated bacteria and the possible genetic adaptations to these different bacteria. Together our results will lead to a more complete understanding of the adaptation and evolution of C. elegans. This work is supported the European Science Foundation (ESF): NEMADAPT - Molecular architecture of environmental adaptation in natural populations of the nematode Caenorhabditis elegans.

M3 - Abstract

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BT - Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012

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Volkers RJM, Snoek LB, Riksen JAG, Schulenburg H, Braeckman B, Kammenga JE. Genomic and phenotypic differences in Caenorhabditis elegans isolated from different habitats. In Abstracts of papers presented at the Evolution of Caenorhabditis and Other Nematodes, Cold Spring Harbor Laboratory, New York, USA, 3-6 April 2012. 2012. p. 92-92