Genome-Wide Gene Expression Analysis in Response to Organophosphorus Pesticide Chlorpyrifos and Diazion in C.Elegans

A. Viñuela Rodriguez, L.B. Snoek, J.A.G. Riksen, J.E. Kammenga

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

pesticides (OPs) were originally designed to affect the nervous system by inhibiting the enzyme acetylcholinesterase, an important regulator of the neurotransmitter acetylcholine. Over the past years evidence is mounting that these compounds affect many other processes. Little is known, however, about gene expression responses against OPs in the nematode Caenorhabditis elegans. This is surprising because C. elegans is extensively used as a model species in toxicity studies. To address this question we performed a microarray study in C. elegans which was exposed for 72 hrs to two widely used Ops, chlorpyrifos and diazinon, and a low dose mixture of these two compounds. Our analysis revealed transcriptional responses related to detoxification, stress, innate immunity, and transport and metabolism of lipids in all treatments. We found that for both compounds as well as in the mixture, these processes were regulated by different gene transcripts. Our results illustrate intense, and unexpected crosstalk between gene pathways in response to chlorpyrifos and diazinon in C. elegans
Original languageEnglish
Article numbere12145
Number of pages8
JournalPLoS ONE
Volume5
Issue number8
DOIs
Publication statusPublished - 2010

Keywords

  • nematode caenorhabditis-elegans
  • binary-mixture
  • microarray data
  • in-vitro
  • toxicity
  • model
  • mechanism
  • rat
  • neurotoxicity
  • bioconductor

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