Abstract
Natural antibodies (NAb) are produced without any antigenic stimulation as a part of the innate immune system and provide a first line of defense against pathogens. Hence, they may be a useful trait when estimating an animal's potential immune competence and in selection for disease resistance. The aim of this study was to identify genomic regions associated with different NAb traits in milk and potentially describe candidate genes. Milk samples from 1,695 first-lactation Holstein Friesian cows with titer measurements for keyhole limpet hemocyanin, lipopolysaccharide, lipoteichoic acid, and peptidoglycan-binding total NAb and isotypes IgG1, IgM, and IgA were used. Genome-wide association study analyses were performed using imputed 777K SNP genotypes, accounting for relationships using pedigree information. Functional enrichment analysis was performed on the significantly associated genomic regions to look for candidate genes. For IgM NAb, significant associations (false discovery rate <0.05) were found on Bos taurus autosome (BTA) 17, 18, and 21 with candidate genes related to immunoglobulin structure and early B cell development. For IgG1, associations were found on BTA3, and we confirmed a quantitative trait loci on BTA21 previously reported for IgG NAb in serum. Our results provide new insights into the regulation of milk NAb that will help unravel the complex relationship between milk immunoglobulins and disease resistance in dairy cattle.
| Original language | English |
|---|---|
| Pages (from-to) | 11092-11103 |
| Journal | Journal of Dairy Science |
| Volume | 102 |
| Issue number | 12 |
| Early online date | 20 Sep 2019 |
| DOIs | |
| Publication status | Published - Dec 2019 |
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Keywords
- dairy cattle
- genome-wide association study
- immunoglobulin
- natural antibody
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Genome-wide association study identifies loci influencing natural antibody titers in milk of Dutch Holstein-Friesian cattle. / Cordero-Solorzano, Juan; Parmentier, Henk K.; Arts, Joop A.J.; van der Poel, Jan; de Koning, Dirk Jan; Bovenhuis, Henk.
In: Journal of Dairy Science, Vol. 102, No. 12, 12.2019, p. 11092-11103.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Genome-wide association study identifies loci influencing natural antibody titers in milk of Dutch Holstein-Friesian cattle
AU - Cordero-Solorzano, Juan
AU - Parmentier, Henk K.
AU - Arts, Joop A.J.
AU - van der Poel, Jan
AU - de Koning, Dirk Jan
AU - Bovenhuis, Henk
PY - 2019/12
Y1 - 2019/12
N2 - Natural antibodies (NAb) are produced without any antigenic stimulation as a part of the innate immune system and provide a first line of defense against pathogens. Hence, they may be a useful trait when estimating an animal's potential immune competence and in selection for disease resistance. The aim of this study was to identify genomic regions associated with different NAb traits in milk and potentially describe candidate genes. Milk samples from 1,695 first-lactation Holstein Friesian cows with titer measurements for keyhole limpet hemocyanin, lipopolysaccharide, lipoteichoic acid, and peptidoglycan-binding total NAb and isotypes IgG1, IgM, and IgA were used. Genome-wide association study analyses were performed using imputed 777K SNP genotypes, accounting for relationships using pedigree information. Functional enrichment analysis was performed on the significantly associated genomic regions to look for candidate genes. For IgM NAb, significant associations (false discovery rate <0.05) were found on Bos taurus autosome (BTA) 17, 18, and 21 with candidate genes related to immunoglobulin structure and early B cell development. For IgG1, associations were found on BTA3, and we confirmed a quantitative trait loci on BTA21 previously reported for IgG NAb in serum. Our results provide new insights into the regulation of milk NAb that will help unravel the complex relationship between milk immunoglobulins and disease resistance in dairy cattle.
AB - Natural antibodies (NAb) are produced without any antigenic stimulation as a part of the innate immune system and provide a first line of defense against pathogens. Hence, they may be a useful trait when estimating an animal's potential immune competence and in selection for disease resistance. The aim of this study was to identify genomic regions associated with different NAb traits in milk and potentially describe candidate genes. Milk samples from 1,695 first-lactation Holstein Friesian cows with titer measurements for keyhole limpet hemocyanin, lipopolysaccharide, lipoteichoic acid, and peptidoglycan-binding total NAb and isotypes IgG1, IgM, and IgA were used. Genome-wide association study analyses were performed using imputed 777K SNP genotypes, accounting for relationships using pedigree information. Functional enrichment analysis was performed on the significantly associated genomic regions to look for candidate genes. For IgM NAb, significant associations (false discovery rate <0.05) were found on Bos taurus autosome (BTA) 17, 18, and 21 with candidate genes related to immunoglobulin structure and early B cell development. For IgG1, associations were found on BTA3, and we confirmed a quantitative trait loci on BTA21 previously reported for IgG NAb in serum. Our results provide new insights into the regulation of milk NAb that will help unravel the complex relationship between milk immunoglobulins and disease resistance in dairy cattle.
KW - dairy cattle
KW - genome-wide association study
KW - immunoglobulin
KW - natural antibody
U2 - 10.3168/jds.2019-16627
DO - 10.3168/jds.2019-16627
M3 - Article
VL - 102
SP - 11092
EP - 11103
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 12
ER -