Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes

M.M. van Oers, M.H.C. Abma-Henkens, E.A. Herniou, J.C.W. de Groot, S. Peters, J.M. Vlak

Research output: Contribution to journalArticleAcademicpeer-review

63 Citations (Scopus)

Abstract

The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39·1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named GECCO. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses
Original languageEnglish
Pages (from-to)2069-2080
JournalJournal of General Virology
Volume86
Issue number7
DOIs
Publication statusPublished - 2005

Keywords

  • nuclear polyhedrosis-virus
  • granulovirus genome
  • mamestra-configurata
  • maximum-likelihood
  • organization
  • protein
  • entomopoxvirus
  • identification
  • search
  • rates

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