Genome sequence of an enhancin gene-rich nucleopolyhedrovirus (NPV) from Agrotis segetum: collinearity with Spodoptera exigua multiple NPV

A.K. Jakubowska, S.A. Peters, J. Ziemnicka, J.M. Vlak, M.M. van Oers

Research output: Contribution to journalArticleAcademicpeer-review

52 Citations (Scopus)

Abstract

The genome sequence of a Polish isolate of Agrotis segetum nucleopolyhedrovirus (AgseNPV-A) was determined and analysed. The circular genome is composed of 147 544 bp and has a G+C content of 45¿7 mol%. It contains 153 putative, non-overlapping open reading frames (ORFs) encoding predicted proteins of more than 50 aa, together making up 89¿8 % of the genome. The remaining 10¿2 % of the DNA constitutes non-coding regions and homologous-repeat regions. One hundred and forty-three AgseNPV-A ORFs are homologues of previously reported baculovirus gene sequences. There are ten unique ORFs and they account for 3 % of the genome in total. All 62 lepidopteran baculovirus genes, including the 29 core baculovirus genes, were found in the AgseNPV-A genome. The gene content and gene order of AgseNPV-A are most similar to those of Spodoptera exigua (Se) multiple NPV and their shared homologous genes are 100 % collinear. Three putative enhancin genes were identified in the AgseNPV-A genome. In phylogenetic analysis, the AgseNPV-A enhancins form a cluster separated from enhancins of the Mamestra species NPVs.
Original languageEnglish
Pages (from-to)537-551
Number of pages15
JournalJournal of General Virology
Volume87
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • nuclear-polyhedrosis-virus
  • occlusion-derived virus
  • granulosis-virus
  • pseudaletia-unipuncta
  • mamestra-configurata
  • lymantria-dispar
  • multicapsid nucleopolyhedrovirus
  • helicoverpa-armigera
  • peritrophic membrane
  • granulovirus genome

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