Genome-scale metabolic models: reconstruction and analysis

G.J. Baart, D.E. Martens

Research output: Chapter in Book/Report/Conference proceedingChapter

26 Citations (Scopus)

Abstract

Metabolism can be defined as the complete set of chemical reactions that occur in living organisms in order to maintain life. Enzymes are the main players in this process as they are responsible for catalyzing the chemical reactions. The enzyme-reaction relationships can be used for the reconstruction of a network of reactions, which leads to a metabolic model of metabolism. A genome-scale metabolic network of chemical reactions that take place inside a living organism is primarily reconstructed from the information that is present in its genome and the literature and involves steps such as functional annotation of the genome, identification of the associated reactions and determination of their stoichiometry, assignment of localization, determination of the biomass composition, estimation of energy requirements, and definition of model constraints. This information can be integrated into a stoichiometric model of metabolism that can be used for detailed analysis of the metabolic potential of the organism using constraint-based modeling approaches and hence is valuable in understanding its metabolic capabilities.
Original languageEnglish
Title of host publicationNeisseria meningitidis
Subtitle of host publicationAdvanced Methods and Protocols
EditorsMyron Christodoulides
PublisherHumana Press
Pages107-126
Volume107
Edition29
ISBN (Electronic)9781617793462
ISBN (Print)9781617793455
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in molecular biology
PublisherSpringer Verlag
ISSN (Print)1064-3745

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    Baart, G. J., & Martens, D. E. (2012). Genome-scale metabolic models: reconstruction and analysis. In M. Christodoulides (Ed.), Neisseria meningitidis: Advanced Methods and Protocols (29 ed., Vol. 107, pp. 107-126). (Methods in molecular biology). Humana Press. https://doi.org/10.1007/978-1-61779-346-2_7