Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation

A.R. Marasek Ciolakowska, M.S. Ramanna, W.A. Laak, J.M. van Tuyl

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch). In our study, genomic in situ hybridization (GISH) has been employed to assess the genome composition in eleven 'Elatior'-begonias hybrids and their ancestor genotypes. Genomic DNA of tuberous Begonia was sonicated to 1-10-kb fragments, labelled by nick translation with digoxigenin-11-dUTP and used as a probe whereas B. socotrana DNA was autoclaved to 100 bp fragments and used as block. The genome of tuberous Begonia was clearly pronounced in 'Elatior'-begonias when the probe concentration was similar to 3.75 ng/mu l (150 ng/slide), with 30 times the excess of B. socotrana blocking DNA and stringency of post hybridization washings at 73% (0.1x SSC at 42A degrees C). In 'Elatior'-begonias hybrids GISH distinguished two groups comprising short (0.6-1.03 mu m in length) and relatively longer chromosomes (1.87-3.88 mu m) which represent B. socotrana and tuberous Begonia genomes, respectively. The number of chromosomes derived from tuberous Begonia ranged from 14 to 56 and for B. socotrana from 7 to 28 which suggest the presence of different ploidy levels in analyzed 'Elatior'-begonia hybrids. Intergenomic recombination has not been detected through GISH in hybrids analyzed. Genomic in situ hybridization turned out to be useful to identify the genome constitution of 'Elatior'-begonia hybrids and thus gain an insight into the origins of these cultivars. This knowledge on the ploidy level and genome composition is essential for further progress in breeding Begonias.
Original languageEnglish
Pages (from-to)273-282
JournalEuphytica
Volume171
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • hordeum-vulgare-l
  • lycopersicon-esculentum
  • chromosome elimination
  • cytogenetic analysis
  • bulbosum l
  • plant dna
  • differentiation
  • extraction
  • nicotiana
  • progenies

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