Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius

Jeffrey A. Rosenfeld; Darryl Reeves; Mercer R. Brugler; Apurva Narechania; Sabrina Simon; Russell Durrett; Jonathan Foox; Kevin Shianna; Michael C. Schatz; Jorge Gandara; Ebrahim Afshinnekoo; Ernest T. Lam; Alex R. Hastie; Saki Chan; Han Cao; Michael Saghbini; Alex Kentsis; Paul J. Planet; Vladyslav Kholodovych; Michael Tessler; Richard Baker; Rob DeSalle; Louis N. Sorkin; Sergios Orestis Kolokotronis; Mark E. Siddall; George Amato; Christopher E. Mason

doi:10.1038/ncomms10164

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius

Jeffrey A. Rosenfeld, Darryl Reeves, Mercer R. Brugler, Apurva Narechania, Sabrina Simon, Russell Durrett, Jonathan Foox, Kevin Shianna, Michael C. Schatz, Jorge Gandara, Ebrahim Afshinnekoo, Ernest T. Lam, Alex R. Hastie, Saki Chan, Han Cao, Michael Saghbini, Alex Kentsis, Paul J. Planet, Vladyslav Kholodovych, Michael TesslerRichard Baker, Rob DeSalle, Louis N. Sorkin, Sergios Orestis Kolokotronis, Mark E. Siddall^*, George Amato, Christopher E. Mason

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

36 Citations (Scopus)

Abstract

The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

Original language	English
Article number	10164
Number of pages	10
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms10164
Publication status	Published - 2016

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Rosenfeld, J. A., Reeves, D., Brugler, M. R., Narechania, A., Simon, S., Durrett, R., Foox, J., Shianna, K., Schatz, M. C., Gandara, J., Afshinnekoo, E., Lam, E. T., Hastie, A. R., Chan, S., Cao, H., Saghbini, M., Kentsis, A., Planet, P. J., Kholodovych, V., ... Mason, C. E. (2016). Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius. Nature Communications, 7, [10164]. https://doi.org/10.1038/ncomms10164

Rosenfeld, Jeffrey A. ; Reeves, Darryl ; Brugler, Mercer R. ; Narechania, Apurva ; Simon, Sabrina ; Durrett, Russell ; Foox, Jonathan ; Shianna, Kevin ; Schatz, Michael C. ; Gandara, Jorge ; Afshinnekoo, Ebrahim ; Lam, Ernest T. ; Hastie, Alex R. ; Chan, Saki ; Cao, Han ; Saghbini, Michael ; Kentsis, Alex ; Planet, Paul J. ; Kholodovych, Vladyslav ; Tessler, Michael ; Baker, Richard ; DeSalle, Rob ; Sorkin, Louis N. ; Kolokotronis, Sergios Orestis ; Siddall, Mark E. ; Amato, George ; Mason, Christopher E. / Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius. In: Nature Communications. 2016 ; Vol. 7.

@article{8c77012214fc4f529459ca6f96587706,

title = "Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius",

abstract = "The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.",

author = "Rosenfeld, {Jeffrey A.} and Darryl Reeves and Brugler, {Mercer R.} and Apurva Narechania and Sabrina Simon and Russell Durrett and Jonathan Foox and Kevin Shianna and Schatz, {Michael C.} and Jorge Gandara and Ebrahim Afshinnekoo and Lam, {Ernest T.} and Hastie, {Alex R.} and Saki Chan and Han Cao and Michael Saghbini and Alex Kentsis and Planet, {Paul J.} and Vladyslav Kholodovych and Michael Tessler and Richard Baker and Rob DeSalle and Sorkin, {Louis N.} and Kolokotronis, {Sergios Orestis} and Siddall, {Mark E.} and George Amato and Mason, {Christopher E.}",

year = "2016",

doi = "10.1038/ncomms10164",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

Rosenfeld, JA, Reeves, D, Brugler, MR, Narechania, A, Simon, S, Durrett, R, Foox, J, Shianna, K, Schatz, MC, Gandara, J, Afshinnekoo, E, Lam, ET, Hastie, AR, Chan, S, Cao, H, Saghbini, M, Kentsis, A, Planet, PJ, Kholodovych, V, Tessler, M, Baker, R, DeSalle, R, Sorkin, LN, Kolokotronis, SO, Siddall, ME, Amato, G & Mason, CE 2016, 'Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius', Nature Communications, vol. 7, 10164. https://doi.org/10.1038/ncomms10164

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius. / Rosenfeld, Jeffrey A.; Reeves, Darryl; Brugler, Mercer R.; Narechania, Apurva; Simon, Sabrina; Durrett, Russell; Foox, Jonathan; Shianna, Kevin; Schatz, Michael C.; Gandara, Jorge; Afshinnekoo, Ebrahim; Lam, Ernest T.; Hastie, Alex R.; Chan, Saki; Cao, Han; Saghbini, Michael; Kentsis, Alex; Planet, Paul J.; Kholodovych, Vladyslav; Tessler, Michael; Baker, Richard; DeSalle, Rob; Sorkin, Louis N.; Kolokotronis, Sergios Orestis; Siddall, Mark E.; Amato, George; Mason, Christopher E.

In: Nature Communications, Vol. 7, 10164, 2016.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Durrett, Russell

AU - Foox, Jonathan

AU - Shianna, Kevin

AU - Schatz, Michael C.

AU - Gandara, Jorge

AU - Afshinnekoo, Ebrahim

AU - Lam, Ernest T.

AU - Hastie, Alex R.

AU - Chan, Saki

AU - Cao, Han

AU - Saghbini, Michael

AU - Kentsis, Alex

AU - Planet, Paul J.

AU - Kholodovych, Vladyslav

AU - Tessler, Michael

AU - Baker, Richard

AU - DeSalle, Rob

AU - Sorkin, Louis N.

AU - Kolokotronis, Sergios Orestis

AU - Siddall, Mark E.

AU - Amato, George

AU - Mason, Christopher E.

PY - 2016

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N2 - The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

AB - The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

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