Genetics of mesophilic citrate metabolizing lactic acid bacteria

S. David

Research output: Thesisexternal PhD, WU


<p>A prerequisite for the stabilization of important features, such as aroma production, in starter strains used in dairy fermentations, is an extensive knowledge of the genetic basis of these properties. In this thesis the genetic basis of citrate metabolism in <em>Lactococcus lactis</em> subsp. <em>lactis</em> var. <em>diacetylactis</em> and <em>Leuconostoc</em> spp. <em></em> are studied and genetic aspects of citrate-fermenting <em>Leuconostoc</em> strains are analyzed.<p>- Chapter II describes the development of a cloning system for <em>Leuconostoc</em> which was necessary due to the lack of natural transformation systems.<p>- Chapter III describes the use of polymerase chain reaction to classify <em>Leuconostoc</em> strains.<p>- Since lactose is the most important carbohydrate used by lactic acid bacteria, knowledge of the genetic basis of the genes involved in lactose metabolism can offer important data concerning gene regulation and expression. Chapters IV and V describe the cloning and expression of the lactose genes of <em>Leuconostoc</em> ssp.<p>- Cloning and functional expression in <em>Escherichia coli</em> of the the citrate permease gene of <em>Lactococcus lactis</em> subsp. <em>lactis</em> var. <em>diacetylactis</em> are described in Chapter VI.<p>- A comparison of the lactococcal citrate permease with the <em>Leuconostoc lactis</em> citrate permease gene is described in Chapter VII.<p>- A summary together with concluding remarks are given in Chapter VIII.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • de Vos, W.M., Promotor
  • Konings, W.N., Promotor, External person
Award date11 Mar 1992
Place of PublicationS.l.
Publication statusPublished - 1992


  • lactobacillus
  • lactic acid bacteria
  • microorganisms
  • genetics
  • heritability


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