Genetic versus antigenic differences among highly pathogenic H5N1 avian influenza A viruses

Consequences for vaccine strain selection

Ben Peeters*, Sylvia Reemers, Jos Dortmans, Erik de Vries, Mart de Jong, Saskia van de Zande, Peter J.M. Rottier, Cornelis A.M. de Haan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Highly pathogenic H5N1 avian influenza A viruses display a remarkable genetic and antigenic diversity. We examined to what extent genetic distances between several H5N1 viruses from different clades correlate with antigenic differences and vaccine performance. H5-specific antisera were generated, and cross-reactivity and antigenic distances between 12 different viruses were determined. In general, antigenic distances increased proportional to genetic distances although notable exceptions were observed. Antigenic distances correlated better with genetic variation in 27 selected, antigenically-relevant H5 residues, than in the complete HA1 domain. Variation in these selected residues could accurately predict the antigenic distances for a novel H5N8 virus. Protection provided by vaccines against heterologous H5N1 challenge viruses indicated that cross-protection also correlates better with genetic variation in the selected antigenically-relevant residues than in complete HA1. When time is limited, variation at these selected residues may be used to accurately predict antigenic distance and vaccine performance.
Original languageEnglish
Pages (from-to)83-93
JournalVirology
Volume503
DOIs
Publication statusPublished - 2017

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Influenza in Birds
Influenza A virus
H5N1 Subtype Influenza A Virus
Vaccines
Cross Protection
Antigenic Variation
Immune Sera
Viruses

Keywords

  • Antigenic distance
  • Genetic variation
  • H5N1
  • Hemagglutinin
  • Influenza A virus
  • Vaccine performance

Cite this

Peeters, Ben ; Reemers, Sylvia ; Dortmans, Jos ; de Vries, Erik ; de Jong, Mart ; van de Zande, Saskia ; Rottier, Peter J.M. ; de Haan, Cornelis A.M. / Genetic versus antigenic differences among highly pathogenic H5N1 avian influenza A viruses : Consequences for vaccine strain selection. In: Virology. 2017 ; Vol. 503. pp. 83-93.
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abstract = "Highly pathogenic H5N1 avian influenza A viruses display a remarkable genetic and antigenic diversity. We examined to what extent genetic distances between several H5N1 viruses from different clades correlate with antigenic differences and vaccine performance. H5-specific antisera were generated, and cross-reactivity and antigenic distances between 12 different viruses were determined. In general, antigenic distances increased proportional to genetic distances although notable exceptions were observed. Antigenic distances correlated better with genetic variation in 27 selected, antigenically-relevant H5 residues, than in the complete HA1 domain. Variation in these selected residues could accurately predict the antigenic distances for a novel H5N8 virus. Protection provided by vaccines against heterologous H5N1 challenge viruses indicated that cross-protection also correlates better with genetic variation in the selected antigenically-relevant residues than in complete HA1. When time is limited, variation at these selected residues may be used to accurately predict antigenic distance and vaccine performance.",
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Genetic versus antigenic differences among highly pathogenic H5N1 avian influenza A viruses : Consequences for vaccine strain selection. / Peeters, Ben; Reemers, Sylvia; Dortmans, Jos; de Vries, Erik; de Jong, Mart; van de Zande, Saskia; Rottier, Peter J.M.; de Haan, Cornelis A.M.

In: Virology, Vol. 503, 2017, p. 83-93.

Research output: Contribution to journalArticleAcademicpeer-review

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