Genetic population differentiation and connectivity among fragmented Moor frog (Rana arvalis) populations in The Netherlands

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Abstract

We studied the effects of landscape structure, habitat loss and fragmentation on genetic differentiation of Moor frog populations in two landscapes in The Netherlands (Drenthe and Noord-Brabant). Microsatellite data of eight loci showed small to moderate genetic differentiation among populations in both landscapes (F ST values 0.022 and 0.060, respectively). Both heterozygosity and population differentiation indicate a lower level of gene flow among populations in Noord-Brabant, where populations were further apart and have experienced a higher degree of fragmentation for a longer period of time as compared to populations in Drenthe. A significant isolation-by-distance pattern was found in Drenthe, indicating a limitation in dispersal among populations due to geographic distance. In Noord-Brabant a similar positive correlation was obtained only after the exclusion of a single long-time isolated population. After randomised exclusion of populations a significant additional negative effect of roads was found but not of other landscape elements. These results are discussed in view of improving methodology of assessing the effects of landscape elements on connectivity.
Original languageEnglish
Pages (from-to)1489-1500
JournalLandscape Ecology
Volume22
Issue number10
DOIs
Publication statusPublished - 2007

Fingerprint

frog
population genetics
connectivity
Netherlands
genetic differentiation
landscape structure
isolated population
habitat loss
habitat fragmentation
heterozygosity
gene flow
fragmentation
exclusion
road
methodology
moor
effect
habitat
social isolation
Values

Keywords

  • landscape genetics
  • flow
  • microsatellite
  • conservation
  • diversity
  • distance
  • markers
  • toad

Cite this

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title = "Genetic population differentiation and connectivity among fragmented Moor frog (Rana arvalis) populations in The Netherlands",
abstract = "We studied the effects of landscape structure, habitat loss and fragmentation on genetic differentiation of Moor frog populations in two landscapes in The Netherlands (Drenthe and Noord-Brabant). Microsatellite data of eight loci showed small to moderate genetic differentiation among populations in both landscapes (F ST values 0.022 and 0.060, respectively). Both heterozygosity and population differentiation indicate a lower level of gene flow among populations in Noord-Brabant, where populations were further apart and have experienced a higher degree of fragmentation for a longer period of time as compared to populations in Drenthe. A significant isolation-by-distance pattern was found in Drenthe, indicating a limitation in dispersal among populations due to geographic distance. In Noord-Brabant a similar positive correlation was obtained only after the exclusion of a single long-time isolated population. After randomised exclusion of populations a significant additional negative effect of roads was found but not of other landscape elements. These results are discussed in view of improving methodology of assessing the effects of landscape elements on connectivity.",
keywords = "landscape genetics, flow, microsatellite, conservation, diversity, distance, markers, toad",
author = "P.F.P. Arens and {van der Sluis}, T. and {van 't Westende}, W.P.C. and B. Vosman and C.C. Vos and M.J.M. Smulders",
year = "2007",
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language = "English",
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TY - JOUR

T1 - Genetic population differentiation and connectivity among fragmented Moor frog (Rana arvalis) populations in The Netherlands

AU - Arens, P.F.P.

AU - van der Sluis, T.

AU - van 't Westende, W.P.C.

AU - Vosman, B.

AU - Vos, C.C.

AU - Smulders, M.J.M.

PY - 2007

Y1 - 2007

N2 - We studied the effects of landscape structure, habitat loss and fragmentation on genetic differentiation of Moor frog populations in two landscapes in The Netherlands (Drenthe and Noord-Brabant). Microsatellite data of eight loci showed small to moderate genetic differentiation among populations in both landscapes (F ST values 0.022 and 0.060, respectively). Both heterozygosity and population differentiation indicate a lower level of gene flow among populations in Noord-Brabant, where populations were further apart and have experienced a higher degree of fragmentation for a longer period of time as compared to populations in Drenthe. A significant isolation-by-distance pattern was found in Drenthe, indicating a limitation in dispersal among populations due to geographic distance. In Noord-Brabant a similar positive correlation was obtained only after the exclusion of a single long-time isolated population. After randomised exclusion of populations a significant additional negative effect of roads was found but not of other landscape elements. These results are discussed in view of improving methodology of assessing the effects of landscape elements on connectivity.

AB - We studied the effects of landscape structure, habitat loss and fragmentation on genetic differentiation of Moor frog populations in two landscapes in The Netherlands (Drenthe and Noord-Brabant). Microsatellite data of eight loci showed small to moderate genetic differentiation among populations in both landscapes (F ST values 0.022 and 0.060, respectively). Both heterozygosity and population differentiation indicate a lower level of gene flow among populations in Noord-Brabant, where populations were further apart and have experienced a higher degree of fragmentation for a longer period of time as compared to populations in Drenthe. A significant isolation-by-distance pattern was found in Drenthe, indicating a limitation in dispersal among populations due to geographic distance. In Noord-Brabant a similar positive correlation was obtained only after the exclusion of a single long-time isolated population. After randomised exclusion of populations a significant additional negative effect of roads was found but not of other landscape elements. These results are discussed in view of improving methodology of assessing the effects of landscape elements on connectivity.

KW - landscape genetics

KW - flow

KW - microsatellite

KW - conservation

KW - diversity

KW - distance

KW - markers

KW - toad

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DO - 10.1007/s10980-007-9132-4

M3 - Article

VL - 22

SP - 1489

EP - 1500

JO - Landscape Ecology

JF - Landscape Ecology

SN - 0921-2973

IS - 10

ER -