Genetic mapping and annotation of genomic microsatellites isolated from globe artichoke

A. Acquadro, S. Lanteri, D. Scaglione, P.F.P. Arens, B. Vosman, E. Portis

    Research output: Contribution to journalArticleAcademicpeer-review

    32 Citations (Scopus)

    Abstract

    Cynara cardunculus includes three taxa, the globe artichoke (subsp. scolymus L. Hegi), the cultivated cardoon (var. altilis) and their progenitor, the wild cardoon (var. sylvestris). Globe artichoke is an important component of the Mediterranean rural economy, but its improvement through breeding has been rather limited and its genome organization remains largely unexplored. Here, we report the isolation of 61 new microsatellite loci which amplified a total of 208 alleles in a panel of 22 C. cardunculus genotypes. Of these, 51 were informative for linkage analysis and 39 were used to increase marker density in the available globe artichoke genetic maps. Sequence analysis of the 22 loci associated with genes showed that 9 are located within coding sequence, with the repetitive domain probably being involved in DNA binding or in protein¿protein interactions. The expression of the genes associated with 9 of the 22 microsatellite loci was demonstrated by RT-PCR.
    Original languageEnglish
    Pages (from-to)1573-1587
    JournalTheoretical and Applied Genetics
    Volume118
    Issue number8
    DOIs
    Publication statusPublished - 2009

    Keywords

    • cynara-cardunculus l.
    • fragment length polymorphisms
    • transcription factor-binding
    • cultured rat hepatocytes
    • linkage map
    • scolymus l.
    • mediterranean environment
    • medicago-truncatula
    • potential source
    • dna-sequences

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