Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.)

Jose Rafael Chan Navarrete, Oene Dolstra, Martijn van Kaauwen, Edith T. Lammerts van Bueren, Gerard van der Linden

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Cultivation of spinach requires high amounts of nitrogen (N), which puts a strain on the environment. A sustainable solution to this problem is to breed for crops with higher N use efficiency (NUE). The aim of this study was to provide tools for molecular breeding and to elucidate the genetic variation of factors contributing to NUE in spinach. A cross was made between two F1 hybrid cultivars contrasting in NUE. Several F1 progeny were self-pollinated and based on evaluation of the F2 generation, a mapping F2 population (335 individuals) of a single F1 was selected. SNP markers for the genetic map were discovered by RNA sequencing of the two parent cultivars, and 283 SNP markers were used to produce a genetic map comprising of six linkage groups (P01–P06), ranging in size from 46 to 116 cM. NUE related traits were determined for a set of F2:3 families grown under low and high N conditions in a hydroponics system under an Ingestad N-addition model. Interval mapping analysis detected 39 trait-specific QTLs, with several QTLs accumulating on P01 and P02 of the linkage map. The QTLs and in particular the P01 and P02 regions provide potential targets for the improvement of NUE in spinach.

Original languageEnglish
Pages (from-to)621-636
JournalEuphytica
Volume208
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

Spinacia oleracea
nutrient use efficiency
spinach
quantitative trait loci
Nitrogen
Single Nucleotide Polymorphism
DNA Shuffling
Hydroponics
RNA Sequence Analysis
cultivars
Genetic Markers
linkage groups
hydroponics
chromosome mapping
sequence analysis
breeds
genetic variation
genetic markers
breeding
nitrogen

Keywords

  • Genetic map
  • Nitrogen use efficiency (NUE)
  • Quantitative trait locus (QTL)
  • Spinacia oleracea

Cite this

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title = "Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.)",
abstract = "Cultivation of spinach requires high amounts of nitrogen (N), which puts a strain on the environment. A sustainable solution to this problem is to breed for crops with higher N use efficiency (NUE). The aim of this study was to provide tools for molecular breeding and to elucidate the genetic variation of factors contributing to NUE in spinach. A cross was made between two F1 hybrid cultivars contrasting in NUE. Several F1 progeny were self-pollinated and based on evaluation of the F2 generation, a mapping F2 population (335 individuals) of a single F1 was selected. SNP markers for the genetic map were discovered by RNA sequencing of the two parent cultivars, and 283 SNP markers were used to produce a genetic map comprising of six linkage groups (P01–P06), ranging in size from 46 to 116 cM. NUE related traits were determined for a set of F2:3 families grown under low and high N conditions in a hydroponics system under an Ingestad N-addition model. Interval mapping analysis detected 39 trait-specific QTLs, with several QTLs accumulating on P01 and P02 of the linkage map. The QTLs and in particular the P01 and P02 regions provide potential targets for the improvement of NUE in spinach.",
keywords = "Genetic map, Nitrogen use efficiency (NUE), Quantitative trait locus (QTL), Spinacia oleracea",
author = "{Chan Navarrete}, {Jose Rafael} and Oene Dolstra and {van Kaauwen}, Martijn and {Lammerts van Bueren}, {Edith T.} and {van der Linden}, Gerard",
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Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.). / Chan Navarrete, Jose Rafael; Dolstra, Oene; van Kaauwen, Martijn; Lammerts van Bueren, Edith T.; van der Linden, Gerard.

In: Euphytica, Vol. 208, No. 3, 2016, p. 621-636.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic map construction and QTL analysis of nitrogen use efficiency in spinach (Spinacia oleracea L.)

AU - Chan Navarrete, Jose Rafael

AU - Dolstra, Oene

AU - van Kaauwen, Martijn

AU - Lammerts van Bueren, Edith T.

AU - van der Linden, Gerard

PY - 2016

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