Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus

Susanne Niedermeyer, Martin Eiden, Pavlos Toumazos, Jan Langeveld, Alex Bossers

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE's) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE's. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrPSc) in the obex. PrPSc deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrPSc-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p <0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies.

Original languageEnglish
Article number99
Number of pages14
JournalVeterinary Research
Volume47
DOIs
Publication statusPublished - 2016

Fingerprint

Cyprus
Scrapie
Prion Diseases
prion diseases
scrapie
Goats
Molecular Biology
goats
PrPSc proteins
prions
Bovine Spongiform Encephalopathy
genetic polymorphism
bovine spongiform encephalopathy
Codon
codons
Sheep
Endopeptidase K
sheep
Peripheral Nervous System
Brain Diseases

Cite this

Niedermeyer, Susanne ; Eiden, Martin ; Toumazos, Pavlos ; Langeveld, Jan ; Bossers, Alex. / Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus. In: Veterinary Research. 2016 ; Vol. 47.
@article{95a3bc0f96104e9d9d0ec862acb7a0c8,
title = "Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus",
abstract = "Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE's) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE's. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrPSc) in the obex. PrPSc deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrPSc-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p <0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies.",
author = "Susanne Niedermeyer and Martin Eiden and Pavlos Toumazos and Jan Langeveld and Alex Bossers",
year = "2016",
doi = "10.1186/s13567-016-0379-0",
language = "English",
volume = "47",
journal = "Veterinary Research",
issn = "0928-4249",
publisher = "Springer Verlag",

}

Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus. / Niedermeyer, Susanne; Eiden, Martin; Toumazos, Pavlos; Langeveld, Jan; Bossers, Alex.

In: Veterinary Research, Vol. 47, 99, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus

AU - Niedermeyer, Susanne

AU - Eiden, Martin

AU - Toumazos, Pavlos

AU - Langeveld, Jan

AU - Bossers, Alex

PY - 2016

Y1 - 2016

N2 - Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE's) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE's. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrPSc) in the obex. PrPSc deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrPSc-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p <0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies.

AB - Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE's) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE's. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrPSc) in the obex. PrPSc deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrPSc-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p <0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies.

U2 - 10.1186/s13567-016-0379-0

DO - 10.1186/s13567-016-0379-0

M3 - Article

VL - 47

JO - Veterinary Research

JF - Veterinary Research

SN - 0928-4249

M1 - 99

ER -