Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments.

M. Kulczyk-Skrzeszewska, M.J.M. Smulders, W.P.C. van 't Westende, N. Glowska, B. Kieliszewska-Rokicka

    Research output: Chapter in Book/Report/Conference proceedingAbstract

    Abstract

    Populus is one of the few tree genera with both ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) associations. Little is known about the factors influencing the composition of mycorrhizal fungal communities associated with poplar trees. The frequent interspecific hybridization of poplars complicates field studies aimed at distinguishing the effects of genetic and environmental factors on mycorrhizal communities. Lombardy poplar (P. nigra L. cv ‘Italica’) is a male clone selected in Lombardy, northern Italy, in the 17th century. Spread by cuttings throughout Europe and the USA it has been cultivated as a street and landscape tree in a wide variety of environments. The genetic similarity of Lombardy poplar trees provides an opportunity to determine the effects of local environmental conditions on the composition of mycorrhizal fungal communities associated with a single tree genotype. We examined mycorrhizae of mature Lombardy poplar trees grown under various soil conditions and subject to various types and levels of anthropogenic pressure in Poland. Fine roots were sampled randomly from the upper soil layer in spring and late summer. ECM and AM colonization of the poplar roots were evaluated using the intersection method after cleaning and staining of the root samples with trypan blue. Ectomycorrhizae were sorted into morphotypes based on their morphological characters. DNA was extracted from individual ectomycorrhizae and the rDNA ITS region of the fungal symbiont was amplified by PCR, cloned and sequenced. Molecular taxonomical identification was accomplished using BLAST analysis against sequences in databases followed by phylogenetic analysis (neighbour joining method) of ITS sequences. Alignment with ITS sequences in various databases showed various species of Tuber, Tomentella and Hebeloma, one species each of Laccaria, Helvella, Paxillus and Thelephora and possibly several other species as well; we obtained a number of sequences with close matches in the EMBL Genbank to ‘uncultivated fungal species’ only. The proportion of ECM and AM colonization and the richness and composition of ECM mycobionts associated with the poplar roots differed between study sites, suggesting an effect of local environmental conditions on mycorrhizal symbiosis
    Original languageEnglish
    Title of host publicationBook Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010
    EditorsB. Vinceti, P. Neate
    Place of PublicationRome en Madrid
    PublisherBiodiversity International
    Pages113-113
    ISBN (Print)9789290438342
    Publication statusPublished - 2010
    EventConference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain -
    Duration: 9 Jun 201011 Jun 2010

    Conference

    ConferenceConference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain
    Period9/06/1011/06/10

    Fingerprint

    Populus nigra
    symbionts
    genetic variation
    ectomycorrhizae
    fungal communities
    environmental factors
    Helvella
    Thelephora
    Paxillus
    Italy
    Laccaria
    Tomentella
    Hebeloma
    interspecific hybridization
    Populus
    mycorrhizae
    cleaning
    symbiosis
    Poland
    soil quality

    Cite this

    Kulczyk-Skrzeszewska, M., Smulders, M. J. M., van 't Westende, W. P. C., Glowska, N., & Kieliszewska-Rokicka, B. (2010). Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments. In B. Vinceti, & P. Neate (Eds.), Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010 (pp. 113-113). Rome en Madrid: Biodiversity International.
    Kulczyk-Skrzeszewska, M. ; Smulders, M.J.M. ; van 't Westende, W.P.C. ; Glowska, N. ; Kieliszewska-Rokicka, B. / Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments. Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010. editor / B. Vinceti ; P. Neate. Rome en Madrid : Biodiversity International, 2010. pp. 113-113
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    title = "Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments.",
    abstract = "Populus is one of the few tree genera with both ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) associations. Little is known about the factors influencing the composition of mycorrhizal fungal communities associated with poplar trees. The frequent interspecific hybridization of poplars complicates field studies aimed at distinguishing the effects of genetic and environmental factors on mycorrhizal communities. Lombardy poplar (P. nigra L. cv ‘Italica’) is a male clone selected in Lombardy, northern Italy, in the 17th century. Spread by cuttings throughout Europe and the USA it has been cultivated as a street and landscape tree in a wide variety of environments. The genetic similarity of Lombardy poplar trees provides an opportunity to determine the effects of local environmental conditions on the composition of mycorrhizal fungal communities associated with a single tree genotype. We examined mycorrhizae of mature Lombardy poplar trees grown under various soil conditions and subject to various types and levels of anthropogenic pressure in Poland. Fine roots were sampled randomly from the upper soil layer in spring and late summer. ECM and AM colonization of the poplar roots were evaluated using the intersection method after cleaning and staining of the root samples with trypan blue. Ectomycorrhizae were sorted into morphotypes based on their morphological characters. DNA was extracted from individual ectomycorrhizae and the rDNA ITS region of the fungal symbiont was amplified by PCR, cloned and sequenced. Molecular taxonomical identification was accomplished using BLAST analysis against sequences in databases followed by phylogenetic analysis (neighbour joining method) of ITS sequences. Alignment with ITS sequences in various databases showed various species of Tuber, Tomentella and Hebeloma, one species each of Laccaria, Helvella, Paxillus and Thelephora and possibly several other species as well; we obtained a number of sequences with close matches in the EMBL Genbank to ‘uncultivated fungal species’ only. The proportion of ECM and AM colonization and the richness and composition of ECM mycobionts associated with the poplar roots differed between study sites, suggesting an effect of local environmental conditions on mycorrhizal symbiosis",
    author = "M. Kulczyk-Skrzeszewska and M.J.M. Smulders and {van 't Westende}, W.P.C. and N. Glowska and B. Kieliszewska-Rokicka",
    year = "2010",
    language = "English",
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    pages = "113--113",
    editor = "B. Vinceti and P. Neate",
    booktitle = "Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010",
    publisher = "Biodiversity International",

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    Kulczyk-Skrzeszewska, M, Smulders, MJM, van 't Westende, WPC, Glowska, N & Kieliszewska-Rokicka, B 2010, Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments. in B Vinceti & P Neate (eds), Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010. Biodiversity International, Rome en Madrid, pp. 113-113, Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9/06/10.

    Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments. / Kulczyk-Skrzeszewska, M.; Smulders, M.J.M.; van 't Westende, W.P.C.; Glowska, N.; Kieliszewska-Rokicka, B.

    Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010. ed. / B. Vinceti; P. Neate. Rome en Madrid : Biodiversity International, 2010. p. 113-113.

    Research output: Chapter in Book/Report/Conference proceedingAbstract

    TY - CHAP

    T1 - Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments.

    AU - Kulczyk-Skrzeszewska, M.

    AU - Smulders, M.J.M.

    AU - van 't Westende, W.P.C.

    AU - Glowska, N.

    AU - Kieliszewska-Rokicka, B.

    PY - 2010

    Y1 - 2010

    N2 - Populus is one of the few tree genera with both ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) associations. Little is known about the factors influencing the composition of mycorrhizal fungal communities associated with poplar trees. The frequent interspecific hybridization of poplars complicates field studies aimed at distinguishing the effects of genetic and environmental factors on mycorrhizal communities. Lombardy poplar (P. nigra L. cv ‘Italica’) is a male clone selected in Lombardy, northern Italy, in the 17th century. Spread by cuttings throughout Europe and the USA it has been cultivated as a street and landscape tree in a wide variety of environments. The genetic similarity of Lombardy poplar trees provides an opportunity to determine the effects of local environmental conditions on the composition of mycorrhizal fungal communities associated with a single tree genotype. We examined mycorrhizae of mature Lombardy poplar trees grown under various soil conditions and subject to various types and levels of anthropogenic pressure in Poland. Fine roots were sampled randomly from the upper soil layer in spring and late summer. ECM and AM colonization of the poplar roots were evaluated using the intersection method after cleaning and staining of the root samples with trypan blue. Ectomycorrhizae were sorted into morphotypes based on their morphological characters. DNA was extracted from individual ectomycorrhizae and the rDNA ITS region of the fungal symbiont was amplified by PCR, cloned and sequenced. Molecular taxonomical identification was accomplished using BLAST analysis against sequences in databases followed by phylogenetic analysis (neighbour joining method) of ITS sequences. Alignment with ITS sequences in various databases showed various species of Tuber, Tomentella and Hebeloma, one species each of Laccaria, Helvella, Paxillus and Thelephora and possibly several other species as well; we obtained a number of sequences with close matches in the EMBL Genbank to ‘uncultivated fungal species’ only. The proportion of ECM and AM colonization and the richness and composition of ECM mycobionts associated with the poplar roots differed between study sites, suggesting an effect of local environmental conditions on mycorrhizal symbiosis

    AB - Populus is one of the few tree genera with both ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) associations. Little is known about the factors influencing the composition of mycorrhizal fungal communities associated with poplar trees. The frequent interspecific hybridization of poplars complicates field studies aimed at distinguishing the effects of genetic and environmental factors on mycorrhizal communities. Lombardy poplar (P. nigra L. cv ‘Italica’) is a male clone selected in Lombardy, northern Italy, in the 17th century. Spread by cuttings throughout Europe and the USA it has been cultivated as a street and landscape tree in a wide variety of environments. The genetic similarity of Lombardy poplar trees provides an opportunity to determine the effects of local environmental conditions on the composition of mycorrhizal fungal communities associated with a single tree genotype. We examined mycorrhizae of mature Lombardy poplar trees grown under various soil conditions and subject to various types and levels of anthropogenic pressure in Poland. Fine roots were sampled randomly from the upper soil layer in spring and late summer. ECM and AM colonization of the poplar roots were evaluated using the intersection method after cleaning and staining of the root samples with trypan blue. Ectomycorrhizae were sorted into morphotypes based on their morphological characters. DNA was extracted from individual ectomycorrhizae and the rDNA ITS region of the fungal symbiont was amplified by PCR, cloned and sequenced. Molecular taxonomical identification was accomplished using BLAST analysis against sequences in databases followed by phylogenetic analysis (neighbour joining method) of ITS sequences. Alignment with ITS sequences in various databases showed various species of Tuber, Tomentella and Hebeloma, one species each of Laccaria, Helvella, Paxillus and Thelephora and possibly several other species as well; we obtained a number of sequences with close matches in the EMBL Genbank to ‘uncultivated fungal species’ only. The proportion of ECM and AM colonization and the richness and composition of ECM mycobionts associated with the poplar roots differed between study sites, suggesting an effect of local environmental conditions on mycorrhizal symbiosis

    M3 - Abstract

    SN - 9789290438342

    SP - 113

    EP - 113

    BT - Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010

    A2 - Vinceti, B.

    A2 - Neate, P.

    PB - Biodiversity International

    CY - Rome en Madrid

    ER -

    Kulczyk-Skrzeszewska M, Smulders MJM, van 't Westende WPC, Glowska N, Kieliszewska-Rokicka B. Genetic diversity of ectomycorrhizal symbionts of Lombardy poplar (Populus nigra L. Cv 'Italica'') grown in a range of environments. In Vinceti B, Neate P, editors, Book Abstracts of the Conference on 'Forest ecosystem genomics and adapation'', San Lorenzo de El Escorial (Madrid) Spain, 9-11 June 2010. Rome en Madrid: Biodiversity International. 2010. p. 113-113