TY - JOUR
T1 - Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile
AU - Sepúlveda-García, Paulina
AU - Jara, Ronald
AU - Mella, Armin
AU - Monti, Gustavo
AU - Canales, Nivia
AU - Furquim, Maria Eduarda Chiaradia
AU - André, Marcos Rogério
AU - Müller, Ananda
PY - 2024/4
Y1 - 2024/4
N2 - The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with Bartonella henselae whereas 9 showed 99.8–100% identity with Bartonella koehlerae. Haplotype analysis yielded 29 different rpoB-B. henselae haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 rpoB-B. koehlerae haplotypes, with hap#2 represented in all 3 B. koehlerae infected cats. More than one rpoB -B. henselae and B. koehlerae haplotypes were identified in individual cats, reporting by first time coinfection by different B. henselae/B. koehlerae rpoB variants in cats from Chile.
AB - The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with Bartonella henselae whereas 9 showed 99.8–100% identity with Bartonella koehlerae. Haplotype analysis yielded 29 different rpoB-B. henselae haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 rpoB-B. koehlerae haplotypes, with hap#2 represented in all 3 B. koehlerae infected cats. More than one rpoB -B. henselae and B. koehlerae haplotypes were identified in individual cats, reporting by first time coinfection by different B. henselae/B. koehlerae rpoB variants in cats from Chile.
KW - Bartonella spp
KW - Cloning
KW - Coinfection
KW - Genetic diversity
U2 - 10.1016/j.cimid.2024.102150
DO - 10.1016/j.cimid.2024.102150
M3 - Article
C2 - 38401221
AN - SCOPUS:85186068894
SN - 0147-9571
VL - 107
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
M1 - 102150
ER -
