Genetic diversity and population structure of Iranian wild Pleurotus eryngii species-complex strains revealed by URP-PCR markers

Mahdi Behnamian, Seyed A. Mohammadi, A.S.M. Sonnenberg, Ebrahim M. Goltapeh, P.M. Hendrickx

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1 Citation (Scopus)

Abstract

In the present study, a set of 68 P. eryngii wild strains collected from nine locations in northwest and west of Iran along with six commercial strains were studied using universal rice primers (URP). The wild strains were isolated from Ferula ovina, F. haussknechtii, Cachrys ferulacea, Kellusia odoratissima and Smyrniopsis aucheri plant species. Eleven URP primers amplified 188 polymorphic fragments. A total of 3, 2 and 7 bands were specific to the strains collected from F. ovina and C. ferulacea plant species and commercial strains, respectively. The highest and lowest polymorphisms were identified in populations B (66.49%) and F (24.47%), respectively. Genetic distances among populations ranged from 0.027 (between populations A and B) to 0.393 (between populations C and F) with an average of 0.210. The closest and furthest wild populations to commercial strains were populations B (0.102) and F (0.234), respectively. Analysis of molecular variance (AMOVA) revealed significant among regions, among populations within regions and within population diversity, whereas within population variation (61.6%) accounted for most of the total molecular variance.
Original languageEnglish
Pages (from-to)1203-1207
JournalJournal of Food, Agriculture & Environment
Volume8
Issue number3&4
Publication statusPublished - 2010

Fingerprint

Pleurotus eryngii
Pleurotus
species complex
population structure
rice
Polymerase Chain Reaction
genetic variation
wild population
Population
polymorphism
Ferula
genetic diversity
plant species
marker
Oryza
Iran
genetic distance
Analysis of Variance
genetic polymorphism
analysis

Keywords

  • rapd analysis
  • mushroom
  • dna
  • polymorphisms
  • amplification
  • primers
  • fungi

Cite this

Behnamian, Mahdi ; Mohammadi, Seyed A. ; Sonnenberg, A.S.M. ; Goltapeh, Ebrahim M. ; Hendrickx, P.M. / Genetic diversity and population structure of Iranian wild Pleurotus eryngii species-complex strains revealed by URP-PCR markers. In: Journal of Food, Agriculture & Environment. 2010 ; Vol. 8, No. 3&4. pp. 1203-1207.
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abstract = "In the present study, a set of 68 P. eryngii wild strains collected from nine locations in northwest and west of Iran along with six commercial strains were studied using universal rice primers (URP). The wild strains were isolated from Ferula ovina, F. haussknechtii, Cachrys ferulacea, Kellusia odoratissima and Smyrniopsis aucheri plant species. Eleven URP primers amplified 188 polymorphic fragments. A total of 3, 2 and 7 bands were specific to the strains collected from F. ovina and C. ferulacea plant species and commercial strains, respectively. The highest and lowest polymorphisms were identified in populations B (66.49{\%}) and F (24.47{\%}), respectively. Genetic distances among populations ranged from 0.027 (between populations A and B) to 0.393 (between populations C and F) with an average of 0.210. The closest and furthest wild populations to commercial strains were populations B (0.102) and F (0.234), respectively. Analysis of molecular variance (AMOVA) revealed significant among regions, among populations within regions and within population diversity, whereas within population variation (61.6{\%}) accounted for most of the total molecular variance.",
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Genetic diversity and population structure of Iranian wild Pleurotus eryngii species-complex strains revealed by URP-PCR markers. / Behnamian, Mahdi; Mohammadi, Seyed A.; Sonnenberg, A.S.M.; Goltapeh, Ebrahim M.; Hendrickx, P.M.

In: Journal of Food, Agriculture & Environment, Vol. 8, No. 3&4, 2010, p. 1203-1207.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic diversity and population structure of Iranian wild Pleurotus eryngii species-complex strains revealed by URP-PCR markers

AU - Behnamian, Mahdi

AU - Mohammadi, Seyed A.

AU - Sonnenberg, A.S.M.

AU - Goltapeh, Ebrahim M.

AU - Hendrickx, P.M.

PY - 2010

Y1 - 2010

N2 - In the present study, a set of 68 P. eryngii wild strains collected from nine locations in northwest and west of Iran along with six commercial strains were studied using universal rice primers (URP). The wild strains were isolated from Ferula ovina, F. haussknechtii, Cachrys ferulacea, Kellusia odoratissima and Smyrniopsis aucheri plant species. Eleven URP primers amplified 188 polymorphic fragments. A total of 3, 2 and 7 bands were specific to the strains collected from F. ovina and C. ferulacea plant species and commercial strains, respectively. The highest and lowest polymorphisms were identified in populations B (66.49%) and F (24.47%), respectively. Genetic distances among populations ranged from 0.027 (between populations A and B) to 0.393 (between populations C and F) with an average of 0.210. The closest and furthest wild populations to commercial strains were populations B (0.102) and F (0.234), respectively. Analysis of molecular variance (AMOVA) revealed significant among regions, among populations within regions and within population diversity, whereas within population variation (61.6%) accounted for most of the total molecular variance.

AB - In the present study, a set of 68 P. eryngii wild strains collected from nine locations in northwest and west of Iran along with six commercial strains were studied using universal rice primers (URP). The wild strains were isolated from Ferula ovina, F. haussknechtii, Cachrys ferulacea, Kellusia odoratissima and Smyrniopsis aucheri plant species. Eleven URP primers amplified 188 polymorphic fragments. A total of 3, 2 and 7 bands were specific to the strains collected from F. ovina and C. ferulacea plant species and commercial strains, respectively. The highest and lowest polymorphisms were identified in populations B (66.49%) and F (24.47%), respectively. Genetic distances among populations ranged from 0.027 (between populations A and B) to 0.393 (between populations C and F) with an average of 0.210. The closest and furthest wild populations to commercial strains were populations B (0.102) and F (0.234), respectively. Analysis of molecular variance (AMOVA) revealed significant among regions, among populations within regions and within population diversity, whereas within population variation (61.6%) accounted for most of the total molecular variance.

KW - rapd analysis

KW - mushroom

KW - dna

KW - polymorphisms

KW - amplification

KW - primers

KW - fungi

M3 - Article

VL - 8

SP - 1203

EP - 1207

JO - Journal of Food, Agriculture & Environment

JF - Journal of Food, Agriculture & Environment

SN - 1459-0255

IS - 3&4

ER -