Genetic characterization of clavibacter michiganensis subsp. Michiganensis population in Turkey

Yusuf Sen, Yesim Aysan, Mustafa Mirik, Duygu Ozdemir, Fien Meijer-Dekens, Jan M. van der Wolf, Richard G.F. Visser, Sjaak van Heusden*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The pathogenic gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al. is the most harmful bacterium to tomatoes in many countries with a cooler climate. Multilocus sequence analysis was performed on five housekeeping genes (bipA, gyrB, kdpA, ligA, and sdhA) and three virulence-related genes (ppaA, chpC, andtomA) todetermine evolutionary relationships and population structure of 108 C. michiganensis subsp. michiganensis strains collected from Turkey between 1996 and 2012. Based on these analyses, we concluded that C. michiganensis subsp. michiganensis in Turkey is highly uniform. However, at least four novel C. michiganensis subsp. michiganensis strains were recently introduced, possibly at the beginning of the 1990s. The singletons might point to additional sources or to strains that have evolved locally in Turkey.
Original languageEnglish
Pages (from-to)300-308
JournalPlant Disease
Volume102
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

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Clavibacter michiganensis subsp. michiganensis
Turkey (country)
Gram-positive bacteria
coolers
population structure
virulence
tomatoes
climate
bacteria
genes

Cite this

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title = "Genetic characterization of clavibacter michiganensis subsp. Michiganensis population in Turkey",
abstract = "The pathogenic gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al. is the most harmful bacterium to tomatoes in many countries with a cooler climate. Multilocus sequence analysis was performed on five housekeeping genes (bipA, gyrB, kdpA, ligA, and sdhA) and three virulence-related genes (ppaA, chpC, andtomA) todetermine evolutionary relationships and population structure of 108 C. michiganensis subsp. michiganensis strains collected from Turkey between 1996 and 2012. Based on these analyses, we concluded that C. michiganensis subsp. michiganensis in Turkey is highly uniform. However, at least four novel C. michiganensis subsp. michiganensis strains were recently introduced, possibly at the beginning of the 1990s. The singletons might point to additional sources or to strains that have evolved locally in Turkey.",
author = "Yusuf Sen and Yesim Aysan and Mustafa Mirik and Duygu Ozdemir and Fien Meijer-Dekens and {van der Wolf}, {Jan M.} and Visser, {Richard G.F.} and {van Heusden}, Sjaak",
year = "2018",
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doi = "10.1094/PDIS-02-17-0276-RE",
language = "English",
volume = "102",
pages = "300--308",
journal = "Plant Disease",
issn = "0191-2917",
publisher = "American Phytopathological Society",
number = "2",

}

Genetic characterization of clavibacter michiganensis subsp. Michiganensis population in Turkey. / Sen, Yusuf; Aysan, Yesim; Mirik, Mustafa; Ozdemir, Duygu; Meijer-Dekens, Fien; van der Wolf, Jan M.; Visser, Richard G.F.; van Heusden, Sjaak.

In: Plant Disease, Vol. 102, No. 2, 01.02.2018, p. 300-308.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic characterization of clavibacter michiganensis subsp. Michiganensis population in Turkey

AU - Sen, Yusuf

AU - Aysan, Yesim

AU - Mirik, Mustafa

AU - Ozdemir, Duygu

AU - Meijer-Dekens, Fien

AU - van der Wolf, Jan M.

AU - Visser, Richard G.F.

AU - van Heusden, Sjaak

PY - 2018/2/1

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AB - The pathogenic gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al. is the most harmful bacterium to tomatoes in many countries with a cooler climate. Multilocus sequence analysis was performed on five housekeeping genes (bipA, gyrB, kdpA, ligA, and sdhA) and three virulence-related genes (ppaA, chpC, andtomA) todetermine evolutionary relationships and population structure of 108 C. michiganensis subsp. michiganensis strains collected from Turkey between 1996 and 2012. Based on these analyses, we concluded that C. michiganensis subsp. michiganensis in Turkey is highly uniform. However, at least four novel C. michiganensis subsp. michiganensis strains were recently introduced, possibly at the beginning of the 1990s. The singletons might point to additional sources or to strains that have evolved locally in Turkey.

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