Genetic characterization of an atypical Schmallenberg virus isolated from the brain of a malformed lamb

M.M. Hulst, J.A. Kortekaas, R.W. van der Hakze-van der Honing, S.A. Vastenhouw, J.B.W.J. Cornelissen, K. van Maanen, A. Bossers, F.L. Harders, N. Stockhofe, W.H.M. van der Poel

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Abstract

A novel orthobunyavirus, named "Schmallenberg virus" (SBV), was first detected in the blood of cattle at the end of the summer in Germany in 2011, and subsequently in late autumn from the brain of a stillborn malformed lamb in The Netherlands. Full genome sequences, including 5' and 3' terminal "panhandle" sequences of the L, M, and S segments of the SBV isolated from lamb brain tissue (named HL1) were determined. In addition, a second SBV strain was isolated from the blood of a dairy cow (named F6) also in The Netherlands. This isolate was passaged on Vero cells, and its genome sequence was determined by next-generation sequencing. Alignments of the two genome sequences revealed 4, 12, and 2 amino acid differences in the open reading frames of the L, M, and S segments, respectively. Eleven of a total of 12 amino acid differences were detected in the M segment encoding the ectodomain of the putative structural glycoprotein Gc. Notably, in the HL1 isolate, positions 737-739 are occupied by isoleucine, arginine, and leucine (IRL), whereas in the majority of other sequenced SBV isolates these positions are occupied by threonine, histidine, and proline, respectively. Moreover, in all sheep, goat, and cattle SBV isolates sequenced and published so far, an IRL sequence was never found. This has brought us to the conclusion that the M segment of the HL1 isolate differed markedly from that of other lamb and cow isolates. Whether this atypical variant resulted from adaptation to the ewe, fetus, or insect vector remains to be investigated.
Original languageEnglish
Pages (from-to)505-514
JournalVirus Genes
Volume47
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Viruses
Brain
Genome
Netherlands
Orthobunyavirus
Insect Vectors
Amino Acids
Vero Cells
Isoleucine
Threonine
Histidine
Proline
Goats
Leucine
Open Reading Frames
Germany
Arginine
Sheep
Fetus

Keywords

  • akabane virus
  • heparan-sulfate
  • cattle
  • protein
  • orthobunyavirus
  • antibodies
  • nss
  • abnormalities
  • netherlands
  • interferon

Cite this

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title = "Genetic characterization of an atypical Schmallenberg virus isolated from the brain of a malformed lamb",
abstract = "A novel orthobunyavirus, named {"}Schmallenberg virus{"} (SBV), was first detected in the blood of cattle at the end of the summer in Germany in 2011, and subsequently in late autumn from the brain of a stillborn malformed lamb in The Netherlands. Full genome sequences, including 5' and 3' terminal {"}panhandle{"} sequences of the L, M, and S segments of the SBV isolated from lamb brain tissue (named HL1) were determined. In addition, a second SBV strain was isolated from the blood of a dairy cow (named F6) also in The Netherlands. This isolate was passaged on Vero cells, and its genome sequence was determined by next-generation sequencing. Alignments of the two genome sequences revealed 4, 12, and 2 amino acid differences in the open reading frames of the L, M, and S segments, respectively. Eleven of a total of 12 amino acid differences were detected in the M segment encoding the ectodomain of the putative structural glycoprotein Gc. Notably, in the HL1 isolate, positions 737-739 are occupied by isoleucine, arginine, and leucine (IRL), whereas in the majority of other sequenced SBV isolates these positions are occupied by threonine, histidine, and proline, respectively. Moreover, in all sheep, goat, and cattle SBV isolates sequenced and published so far, an IRL sequence was never found. This has brought us to the conclusion that the M segment of the HL1 isolate differed markedly from that of other lamb and cow isolates. Whether this atypical variant resulted from adaptation to the ewe, fetus, or insect vector remains to be investigated.",
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author = "M.M. Hulst and J.A. Kortekaas and {van der Hakze-van der Honing}, R.W. and S.A. Vastenhouw and J.B.W.J. Cornelissen and {van Maanen}, K. and A. Bossers and F.L. Harders and N. Stockhofe and {van der Poel}, W.H.M.",
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journal = "Virus Genes",
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Genetic characterization of an atypical Schmallenberg virus isolated from the brain of a malformed lamb. / Hulst, M.M.; Kortekaas, J.A.; van der Hakze-van der Honing, R.W.; Vastenhouw, S.A.; Cornelissen, J.B.W.J.; van Maanen, K.; Bossers, A.; Harders, F.L.; Stockhofe, N.; van der Poel, W.H.M.

In: Virus Genes, Vol. 47, No. 3, 2013, p. 505-514.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic characterization of an atypical Schmallenberg virus isolated from the brain of a malformed lamb

AU - Hulst, M.M.

AU - Kortekaas, J.A.

AU - van der Hakze-van der Honing, R.W.

AU - Vastenhouw, S.A.

AU - Cornelissen, J.B.W.J.

AU - van Maanen, K.

AU - Bossers, A.

AU - Harders, F.L.

AU - Stockhofe, N.

AU - van der Poel, W.H.M.

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AB - A novel orthobunyavirus, named "Schmallenberg virus" (SBV), was first detected in the blood of cattle at the end of the summer in Germany in 2011, and subsequently in late autumn from the brain of a stillborn malformed lamb in The Netherlands. Full genome sequences, including 5' and 3' terminal "panhandle" sequences of the L, M, and S segments of the SBV isolated from lamb brain tissue (named HL1) were determined. In addition, a second SBV strain was isolated from the blood of a dairy cow (named F6) also in The Netherlands. This isolate was passaged on Vero cells, and its genome sequence was determined by next-generation sequencing. Alignments of the two genome sequences revealed 4, 12, and 2 amino acid differences in the open reading frames of the L, M, and S segments, respectively. Eleven of a total of 12 amino acid differences were detected in the M segment encoding the ectodomain of the putative structural glycoprotein Gc. Notably, in the HL1 isolate, positions 737-739 are occupied by isoleucine, arginine, and leucine (IRL), whereas in the majority of other sequenced SBV isolates these positions are occupied by threonine, histidine, and proline, respectively. Moreover, in all sheep, goat, and cattle SBV isolates sequenced and published so far, an IRL sequence was never found. This has brought us to the conclusion that the M segment of the HL1 isolate differed markedly from that of other lamb and cow isolates. Whether this atypical variant resulted from adaptation to the ewe, fetus, or insect vector remains to be investigated.

KW - akabane virus

KW - heparan-sulfate

KW - cattle

KW - protein

KW - orthobunyavirus

KW - antibodies

KW - nss

KW - abnormalities

KW - netherlands

KW - interferon

U2 - 10.1007/s11262-013-0975-2

DO - 10.1007/s11262-013-0975-2

M3 - Article

VL - 47

SP - 505

EP - 514

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

IS - 3

ER -