Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis

M. Gueimonde; A.B. Florez; A.H.A.M. van Hoek; B. Stuer-Lauridsen; P. Stroman; C.G. de los Reyes-Gavilan; A. Margolles

doi:10.1128/AEM.03096-09

Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis

M. Gueimonde, A.B. Florez, A.H.A.M. van Hoek, B. Stuer-Lauridsen, P. Stroman, C.G. de los Reyes-Gavilan, A. Margolles

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet(W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet(W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis.

Original language	English
Pages (from-to)	3364-3369
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	10
DOIs	https://doi.org/10.1128/AEM.03096-09
Publication status	Published - 2010

Keywords

antibiotic susceptibility
probiotic products
tet(w)
bacteria
bile
lactobacillus
sequences
strains
humans
thermophilum

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10.1128/AEM.03096-09

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title = "Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis",

abstract = "All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet(W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet(W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis.",

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author = "M. Gueimonde and A.B. Florez and {van Hoek}, A.H.A.M. and B. Stuer-Lauridsen and P. Stroman and {de los Reyes-Gavilan}, C.G. and A. Margolles",

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T1 - Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis

AU - Gueimonde, M.

AU - Florez, A.B.

AU - van Hoek, A.H.A.M.

AU - Stuer-Lauridsen, B.

AU - Stroman, P.

AU - de los Reyes-Gavilan, C.G.

AU - Margolles, A.

N1 - ISI:000277388200034

PY - 2010

Y1 - 2010

N2 - All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet(W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet(W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis.

AB - All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet(W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet(W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis.

KW - antibiotic susceptibility

KW - probiotic products

KW - tet(w)

KW - bacteria

KW - bile

KW - lactobacillus

KW - sequences

KW - strains

KW - humans

KW - thermophilum

U2 - 10.1128/AEM.03096-09

DO - 10.1128/AEM.03096-09

M3 - Article

SN - 0099-2240

VL - 76

SP - 3364

EP - 3369

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis

Abstract

Keywords

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