Abstract

Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.
Original languageEnglish
Pages (from-to)1346-1362
JournalNew Phytologist
Volume213
Issue number3
DOIs
Publication statusPublished - 2017

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plant stress
stress tolerance
chromosome mapping
Genome
single nucleotide polymorphism
abiotic stress
genome
quantitative trait loci
stress response
Single Nucleotide Polymorphism
biotic stress
genetic techniques and protocols
plant response
Quantitative Trait Loci
Genome-Wide Association Study
Arabidopsis thaliana
Arabidopsis
phenotype

Cite this

@article{87c27619a5bc4d44a7d045568ee78049,
title = "Genetic architecture of plant stress resistance: multi-trait genome-wide association mapping",
abstract = "Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.",
author = "H.P.M. Thoen and {Davila Olivas}, N.H. and K.J. Kloth and Silvia Coolen and P. Huang and M.G.M. Aarts and J.A. Molenaar and J. Bakker and H.J. Bouwmeester and C. Broekgaarden and J. Bucher and J. Busscher-Lange and X. Cheng and {van Dijk-Fradin}, E.F. and M.A. Jongsma and Julkowska, {Magdalena M.} and J.J.B. Keurentjes and W. Ligterink and Pieterse, {Corn{\'e} M.J.} and C.P. Ruyter-Spira and G. Smant and {van Schaik}, C.C. and {van Wees}, {Saskia C.M.} and R.G.F. Visser and R.E. Voorrips and B. Vosman and D. Vreugdenhil and S. Warmerdam and G.L. Wiegers and {van Heerwaarden}, J. and W.T. Kruijer and {van Eeuwijk}, F.A. and M. Dicke",
year = "2017",
doi = "10.1111/nph.14220",
language = "English",
volume = "213",
pages = "1346--1362",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "3",

}

Genetic architecture of plant stress resistance: multi-trait genome-wide association mapping. / Thoen, H.P.M.; Davila Olivas, N.H.; Kloth, K.J.; Coolen, Silvia; Huang, P.; Aarts, M.G.M.; Molenaar, J.A.; Bakker, J.; Bouwmeester, H.J.; Broekgaarden, C.; Bucher, J.; Busscher-Lange, J.; Cheng, X.; van Dijk-Fradin, E.F.; Jongsma, M.A.; Julkowska, Magdalena M.; Keurentjes, J.J.B.; Ligterink, W.; Pieterse, Corné M.J.; Ruyter-Spira, C.P.; Smant, G.; van Schaik, C.C.; van Wees, Saskia C.M.; Visser, R.G.F.; Voorrips, R.E.; Vosman, B.; Vreugdenhil, D.; Warmerdam, S.; Wiegers, G.L.; van Heerwaarden, J.; Kruijer, W.T.; van Eeuwijk, F.A.; Dicke, M.

In: New Phytologist, Vol. 213, No. 3, 2017, p. 1346-1362.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Genetic architecture of plant stress resistance: multi-trait genome-wide association mapping

AU - Thoen, H.P.M.

AU - Davila Olivas, N.H.

AU - Kloth, K.J.

AU - Coolen, Silvia

AU - Huang, P.

AU - Aarts, M.G.M.

AU - Molenaar, J.A.

AU - Bakker, J.

AU - Bouwmeester, H.J.

AU - Broekgaarden, C.

AU - Bucher, J.

AU - Busscher-Lange, J.

AU - Cheng, X.

AU - van Dijk-Fradin, E.F.

AU - Jongsma, M.A.

AU - Julkowska, Magdalena M.

AU - Keurentjes, J.J.B.

AU - Ligterink, W.

AU - Pieterse, Corné M.J.

AU - Ruyter-Spira, C.P.

AU - Smant, G.

AU - van Schaik, C.C.

AU - van Wees, Saskia C.M.

AU - Visser, R.G.F.

AU - Voorrips, R.E.

AU - Vosman, B.

AU - Vreugdenhil, D.

AU - Warmerdam, S.

AU - Wiegers, G.L.

AU - van Heerwaarden, J.

AU - Kruijer, W.T.

AU - van Eeuwijk, F.A.

AU - Dicke, M.

PY - 2017

Y1 - 2017

N2 - Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.

AB - Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.

U2 - 10.1111/nph.14220

DO - 10.1111/nph.14220

M3 - Article

VL - 213

SP - 1346

EP - 1362

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -