TY - JOUR
T1 - Genetic analysis of morphological traits in a new, versatile, rapid-cycling Brassica rapa recombinant inbred line population
AU - Bagheri, H.
AU - El-Soda, M.
AU - van Oorschot, I.
AU - Hanhart, C.J.
AU - Bonnema, A.B.
AU - Jansen-van den Bosch, T.
AU - Mank, R.
AU - Keurentjes, J.J.B.
AU - Meng, L.
AU - Wu, Jian
AU - Koornneef, M.
AU - Aarts, M.G.M.
PY - 2012/8/16
Y1 - 2012/8/16
N2 - A recombinant inbred line (RIL) population was produced based on a wide cross between the rapid-cycling and self-compatible genotypes L58, a Caixin vegetable type, and R-o-18, a yellow sarson oil type. A linkage map based on 160 F7 lines was constructed using 100 Single nucleotide polymorphisms (SNPs), 130 AFLP®, 27 InDel, and 13 publicly available SSR markers. The map covers a total length of 1150 centiMorgan (cM) with an average resolution of 4.3 cM/marker. To demonstrate the versatility of this new population, 17 traits, related to plant architecture and seed characteristics, were subjected to quantitative trait loci (QTL) analysis. A total of 47 QTLs were detected, each explaining between 6 and 54% of the total phenotypic variance for the concerned trait. The genetic analysis shows that this population is a useful new tool for analyzing genetic variation for interesting traits in B. rapa, and for further exploitation of the recent availability of the B. rapa whole genome sequence for gene cloning and gene function analysis.
AB - A recombinant inbred line (RIL) population was produced based on a wide cross between the rapid-cycling and self-compatible genotypes L58, a Caixin vegetable type, and R-o-18, a yellow sarson oil type. A linkage map based on 160 F7 lines was constructed using 100 Single nucleotide polymorphisms (SNPs), 130 AFLP®, 27 InDel, and 13 publicly available SSR markers. The map covers a total length of 1150 centiMorgan (cM) with an average resolution of 4.3 cM/marker. To demonstrate the versatility of this new population, 17 traits, related to plant architecture and seed characteristics, were subjected to quantitative trait loci (QTL) analysis. A total of 47 QTLs were detected, each explaining between 6 and 54% of the total phenotypic variance for the concerned trait. The genetic analysis shows that this population is a useful new tool for analyzing genetic variation for interesting traits in B. rapa, and for further exploitation of the recent availability of the B. rapa whole genome sequence for gene cloning and gene function analysis.
KW - Brassica rapa
KW - Plant breeding
KW - QTL analysis
KW - Recombinant inbred line population
U2 - 10.3389/fpls.2012.00183
DO - 10.3389/fpls.2012.00183
M3 - Article
SN - 1664-462X
VL - 3
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 183
ER -