Genetic analysis of inbreeding of two strains of the parasitic nematode Haemonchus contortus

M.H. Roos, M. Otsen, R. Hoekstra, J.G. Veenstra, J.A. Lenstra

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    70 Citations (Scopus)


    Haemonchus contortus is a sheep parasitic nematode that causes severe economic losses. Previous studies have indicated a high degree of genetic heterogeneity, which is hardly affected by selection for drug resistance. As a tool for the analysis of the population dynamics of H. contortus and its response to drug resistance, we designed a strategy to study the inbreeding of a benzimidazole-sensitive and a benzimidazole-resistant strain. After 15 generations, a theoretical inbreeding coefficient of 0.87 was achieved. The different stages of inbreeding were analysed using restriction fragment polymorphism, microsatellite variability and amplified fragment length polymorphism. Model-based clustering of the amplified fragment length polymorphism genotypes showed that the allele frequencies of the benzimidazole-resistant strain were stable during the last eight generations. In the sensitive strain a gradual shift of allele frequencies was observed, which led to a temporary increase of the genetic diversity around the eight generations.
    Original languageEnglish
    Pages (from-to)109-115
    JournalInternational Journal for Parasitology
    Issue number1
    Publication statusPublished - 2004


    • beta-tubulin isotype-1
    • benzimidazole resistance
    • anthelmintic resistance
    • selection
    • markers
    • sheep
    • polymorphism
    • variability
    • diversity

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