Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population

H. Bagheri, M. El Soda, H.K. Kim, S. Fritsche, C. Jung, M.G.M. Aarts

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (-, -, -, -, -/- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%-35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.
Original languageEnglish
Pages (from-to)15561-15577
JournalInternational Journal of Molecular Sciences
Volume14
Issue number8
DOIs
Publication statusPublished - 2013

Keywords

  • synechocystis sp pcc-6803
  • quantitative trait loci
  • vitamin-e content
  • arabidopsis-thaliana
  • hydroxyphenylpyruvate dioxygenase
  • tocopherol biosynthesis
  • plastoquinone synthesis
  • functional-analysis
  • natural variation
  • alpha-tocopherol

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