Abstract
This paper presents a novel surface engineering approach that combines photochemical grafting and surface-initiated atom transfer radical polymerization (SI-ATRP) to attach zwitterionic polymer brushes onto indium tin oxide (ITO) substrates. The photochemically grafted hydroxyl-terminated organic layer serves as an excellent platform for initiator attachment, and the zwitterionic polymer generated via subsequent SI-ATRP exhibits very good antifouling properties. Patterned polymer coatings can be obtained when the surface with covalently attached initiator was subjected to photomasked UV-irradiation, in which the C–Br bond that is present in the initiator was broken upon exposure to UV light. A further, highly versatile top-functionalization of the zwitterionic polymer brush was achieved by a strain-promoted alkyne–azide cycloaddition, without compromising its antifouling property. The attached bioligand (here: biotin) enables the specific immobilization of target proteins in a spatially confined fashion, pointing to future applications of this approach in the design of micropatterned sensing platforms on ITO substrates
| Original language | English |
|---|---|
| Pages (from-to) | 12509-12517 |
| Journal | Langmuir |
| Volume | 28 |
| Issue number | 34 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- transfer radical polymerization
- click chemistry
- poly(ethylene glycol)
- protein adsorption
- thin-film
- surfaces
- monolayers
- coatings
- biofunctionalization
- nanoparticles