Generation of tools for characterization of adaptive immune response in carp

Research output: Contribution to journalAbstractAcademic

Abstract

Recently our group reported the development of an intramuscular DNA vaccine against Spring Viremia of Carp Virus (SVCV). This vaccine conferred up to 100% protection to carp even at a single dose of 0.1 μg DNA/g of fish. A further characterization of the adaptive immune response revealed the presence of serum neutralizing antibodies and of memory T cells in vaccine-protected animals. Nevertheless, with the current tools, it was not possible to determine which specific type of immunoglobulin (IgM, IgD, IgT1, IgT2 or all) contributed to the serum neutralizing titres, nor which specific subtype of T cells (CD4+, CD8α+ or both) contributed to the memory response.
In this study, we report the preliminary characterization of tools against B and t cells of carp. For example, we have developed polyclonal antibodies recognizing IgT1+ positive B cells and are further characterizing a monoclonal antibody recognizing putative mucosal T cells. These tools, combined with those already available in our group against IgM+ B cells, CD4-1+ and CD8a+ T cells, provide a panel of markers that will allow the characterization of B and T cell adaptive immune response in carp in general and to vaccination in particular.
Original languageEnglish
Pages (from-to)420-420
JournalFish and Shellfish Immunology
Volume91
DOIs
Publication statusPublished - 2019
EventInternational Fish & Shellfish Immunology Congress 2019 - Gran Canaria, Spain
Duration: 16 Jun 201920 Jun 2019

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T-cells
vaccine
immune response
carp
antibody
T-lymphocytes
serum
B-lymphocytes
DNA
vaccination
Immunoglobulin M
Vaccines
Spring viraemia of carp virus
Cells
vaccines
virus
Data storage equipment
Immunoglobulin D
DNA Vaccines
recombinant vaccines

Cite this

@article{0eb3d5e1813f4a3b99fac5f57e696bd3,
title = "Generation of tools for characterization of adaptive immune response in carp",
abstract = "Recently our group reported the development of an intramuscular DNA vaccine against Spring Viremia of Carp Virus (SVCV). This vaccine conferred up to 100{\%} protection to carp even at a single dose of 0.1 μg DNA/g of fish. A further characterization of the adaptive immune response revealed the presence of serum neutralizing antibodies and of memory T cells in vaccine-protected animals. Nevertheless, with the current tools, it was not possible to determine which specific type of immunoglobulin (IgM, IgD, IgT1, IgT2 or all) contributed to the serum neutralizing titres, nor which specific subtype of T cells (CD4+, CD8α+ or both) contributed to the memory response.In this study, we report the preliminary characterization of tools against B and t cells of carp. For example, we have developed polyclonal antibodies recognizing IgT1+ positive B cells and are further characterizing a monoclonal antibody recognizing putative mucosal T cells. These tools, combined with those already available in our group against IgM+ B cells, CD4-1+ and CD8a+ T cells, provide a panel of markers that will allow the characterization of B and T cell adaptive immune response in carp in general and to vaccination in particular.",
author = "Eltijani Awatif and C.W.E. Embregts and S. Brugman and G. Wiegertjes and M. Forlenza",
year = "2019",
doi = "10.1016/j.fsi.2019.04.308",
language = "English",
volume = "91",
pages = "420--420",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Elsevier",

}

Generation of tools for characterization of adaptive immune response in carp. / Awatif, Eltijani; Embregts, C.W.E.; Brugman, S.; Wiegertjes, G.; Forlenza, M.

In: Fish and Shellfish Immunology, Vol. 91, 2019, p. 420-420.

Research output: Contribution to journalAbstractAcademic

TY - JOUR

T1 - Generation of tools for characterization of adaptive immune response in carp

AU - Awatif, Eltijani

AU - Embregts, C.W.E.

AU - Brugman, S.

AU - Wiegertjes, G.

AU - Forlenza, M.

PY - 2019

Y1 - 2019

N2 - Recently our group reported the development of an intramuscular DNA vaccine against Spring Viremia of Carp Virus (SVCV). This vaccine conferred up to 100% protection to carp even at a single dose of 0.1 μg DNA/g of fish. A further characterization of the adaptive immune response revealed the presence of serum neutralizing antibodies and of memory T cells in vaccine-protected animals. Nevertheless, with the current tools, it was not possible to determine which specific type of immunoglobulin (IgM, IgD, IgT1, IgT2 or all) contributed to the serum neutralizing titres, nor which specific subtype of T cells (CD4+, CD8α+ or both) contributed to the memory response.In this study, we report the preliminary characterization of tools against B and t cells of carp. For example, we have developed polyclonal antibodies recognizing IgT1+ positive B cells and are further characterizing a monoclonal antibody recognizing putative mucosal T cells. These tools, combined with those already available in our group against IgM+ B cells, CD4-1+ and CD8a+ T cells, provide a panel of markers that will allow the characterization of B and T cell adaptive immune response in carp in general and to vaccination in particular.

AB - Recently our group reported the development of an intramuscular DNA vaccine against Spring Viremia of Carp Virus (SVCV). This vaccine conferred up to 100% protection to carp even at a single dose of 0.1 μg DNA/g of fish. A further characterization of the adaptive immune response revealed the presence of serum neutralizing antibodies and of memory T cells in vaccine-protected animals. Nevertheless, with the current tools, it was not possible to determine which specific type of immunoglobulin (IgM, IgD, IgT1, IgT2 or all) contributed to the serum neutralizing titres, nor which specific subtype of T cells (CD4+, CD8α+ or both) contributed to the memory response.In this study, we report the preliminary characterization of tools against B and t cells of carp. For example, we have developed polyclonal antibodies recognizing IgT1+ positive B cells and are further characterizing a monoclonal antibody recognizing putative mucosal T cells. These tools, combined with those already available in our group against IgM+ B cells, CD4-1+ and CD8a+ T cells, provide a panel of markers that will allow the characterization of B and T cell adaptive immune response in carp in general and to vaccination in particular.

U2 - 10.1016/j.fsi.2019.04.308

DO - 10.1016/j.fsi.2019.04.308

M3 - Abstract

VL - 91

SP - 420

EP - 420

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -