Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Gustavo Valbuena; Barry Rockx; Olivier Escaffre

doi:10.1007/978-1-0716-3283-3_14

Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Gustavo Valbuena, Barry Rockx, Olivier Escaffre^*

^*Corresponding author for this work

Virology & Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

The development of humanized mouse models has recently opened new avenues in the field of infectious diseases. These models allow research on many human viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (NSG) mice by the bone marrow, liver, and thymus (BLT) reconstitution method as well as the process of human lung engraftment. We then describe how to infect these human lung grafts with the paramyxovirus Nipah virus (NiV) that can cause lethal respiratory disease in humans, and for which there is only limited understanding of pathogenesis to acute lung injury.

Original language	English
Title of host publication	Nipah Virus
Subtitle of host publication	Methods and Protocols
Editors	A.N. Freiberg, B. Rockx
Place of Publication	New York
Publisher	Humana Press
Pages	191-204
Number of pages	14
ISBN (Electronic)	9781071632833
ISBN (Print)	9781071632826, 9781071632857
DOIs	https://doi.org/10.1007/978-1-0716-3283-3_14
Publication status	Published - 24 Aug 2023

Publication series

Name	Methods in Molecular Biology
Volume	2682
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

BLT reconstitution
Henipavirus
Human lung xenograft
Humanized mouse model
Immune response
NSG mice

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis",

abstract = "The development of humanized mouse models has recently opened new avenues in the field of infectious diseases. These models allow research on many human viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice by the bone marrow, liver, and thymus (BLT) reconstitution method as well as the process of human lung engraftment. We then describe how to infect these human lung grafts with the paramyxovirus Nipah virus (NiV) that can cause lethal respiratory disease in humans, and for which there is only limited understanding of pathogenesis to acute lung injury.",

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author = "Gustavo Valbuena and Barry Rockx and Olivier Escaffre",

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Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis. / Valbuena, Gustavo; Rockx, Barry; Escaffre, Olivier.
Nipah Virus: Methods and Protocols. ed. / A.N. Freiberg; B. Rockx. New York: Humana Press, 2023. p. 191-204 (Methods in Molecular Biology; Vol. 2682).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

AU - Valbuena, Gustavo

AU - Rockx, Barry

AU - Escaffre, Olivier

PY - 2023/8/24

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N2 - The development of humanized mouse models has recently opened new avenues in the field of infectious diseases. These models allow research on many human viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice by the bone marrow, liver, and thymus (BLT) reconstitution method as well as the process of human lung engraftment. We then describe how to infect these human lung grafts with the paramyxovirus Nipah virus (NiV) that can cause lethal respiratory disease in humans, and for which there is only limited understanding of pathogenesis to acute lung injury.

AB - The development of humanized mouse models has recently opened new avenues in the field of infectious diseases. These models allow research on many human viruses that were once difficult to study, because finding suitable animal models of infection can be challenging, cost prohibitive, and often do not entirely recapitulate all parameters of the disease. Here, we describe the procedure of human immune system reconstitution (humanization) of NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice by the bone marrow, liver, and thymus (BLT) reconstitution method as well as the process of human lung engraftment. We then describe how to infect these human lung grafts with the paramyxovirus Nipah virus (NiV) that can cause lethal respiratory disease in humans, and for which there is only limited understanding of pathogenesis to acute lung injury.

KW - BLT reconstitution

KW - Henipavirus

KW - Human lung xenograft

KW - Humanized mouse model

KW - Immune response

KW - NSG mice

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DO - 10.1007/978-1-0716-3283-3_14

M3 - Chapter

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AN - SCOPUS:85168780913

SN - 9781071632826

SN - 9781071632857

T3 - Methods in Molecular Biology

SP - 191

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BT - Nipah Virus

A2 - Freiberg, A.N.

A2 - Rockx, B.

PB - Humana Press

CY - New York

ER -

Generation and Characterization of a Humanized Lung Xenograft Mouse Model for Studying Henipavirus Pathogenesis

Abstract

Publication series

Keywords

UN SDGs

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