General genotyping of Myzus persicae using PCR with microsatellite markers and gel electrophoresis

This protocol can be used to 'roughly' genotype green peach aphids, Myzus persicae (Sulzer), based on microsatellites using PCR and gel electrophoresis with high percentage agarose gels. Please note this protocol does not result in exact allele information as it uses gel-electrophoresis for allele identification and comparisons instead of capillary-electrophoresis. Therefore, allele sizes are judged by eye and thus results are prone to human error as differentiating between identical and different-length alleles might be a challenge.