TY - JOUR
T1 - Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii
AU - de Jaeger, L.
AU - Springer, J.
AU - Wolbert, E.J.H.
AU - Martens, D.E.
AU - Eggink, G.
AU - Wijffels, R.H.
PY - 2017/12
Y1 - 2017/12
N2 - In this study, stearoyl-ACP desaturase (SAD), the enzyme that converts stearic acid into oleic acid, is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii. Two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase, were tested. The mRNA levels for SAD were reduced after the silencing construct was induced. In one of the strains, the reduction in SAD mRNA resulted in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible.
AB - In this study, stearoyl-ACP desaturase (SAD), the enzyme that converts stearic acid into oleic acid, is silenced by artificial microRNA in the green microalga Chlamydomonas reinhardtii. Two different constructs, which target different positions on the mRNA of stearoyl-ACP desaturase, were tested. The mRNA levels for SAD were reduced after the silencing construct was induced. In one of the strains, the reduction in SAD mRNA resulted in a doubling of the stearic acid content in triacylglycerol molecules, which shows that stearic acid production in microalgae is possible.
KW - Chlamydomonas reinhardtii
KW - Enhanced stearic acid
KW - RNA silencing
U2 - 10.1016/j.biortech.2017.06.128
DO - 10.1016/j.biortech.2017.06.128
M3 - Article
AN - SCOPUS:85021856424
SN - 0960-8524
VL - 245
SP - 1616
EP - 1626
JO - Bioresource Technology
JF - Bioresource Technology
IS - B
ER -