Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity

Dalia Alves Carvalho, Marta W. Vasconcelos, Sangseok Lee, Carole F.S. Koning-Boucoiran, Dick Vreugdenhil, Frans A. Krens, Ep Heuvelink, Susana Pinto de Carvalho

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.

Original languageEnglish
Pages (from-to)154-163
JournalPlant Science
Volume253
DOIs
Publication statusPublished - 2016

Fingerprint

Humidity
Abscisic Acid
plant response
humidity
Rosa
Air
Genotype
Gene Expression
abscisic acid
air
gene expression
genotype
Genes
genes
Water
Dehydration
stomatal conductance
stress response
water stress
water

Keywords

  • Abscisic acid
  • Stomatal anatomy
  • Tetraploid roses
  • Vapor pressure deficit
  • Water loss

Cite this

Alves Carvalho, Dalia ; Vasconcelos, Marta W. ; Lee, Sangseok ; Koning-Boucoiran, Carole F.S. ; Vreugdenhil, Dick ; Krens, Frans A. ; Heuvelink, Ep ; Pinto de Carvalho, Susana. / Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity. In: Plant Science. 2016 ; Vol. 253. pp. 154-163.
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title = "Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity",
abstract = "High relative air humidity (RH ≥ 85{\%}) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3{\%}) or high (89 ± 4{\%}) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.",
keywords = "Abscisic acid, Stomatal anatomy, Tetraploid roses, Vapor pressure deficit, Water loss",
author = "{Alves Carvalho}, Dalia and Vasconcelos, {Marta W.} and Sangseok Lee and Koning-Boucoiran, {Carole F.S.} and Dick Vreugdenhil and Krens, {Frans A.} and Ep Heuvelink and {Pinto de Carvalho}, Susana",
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Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity. / Alves Carvalho, Dalia; Vasconcelos, Marta W.; Lee, Sangseok; Koning-Boucoiran, Carole F.S.; Vreugdenhil, Dick; Krens, Frans A.; Heuvelink, Ep; Pinto de Carvalho, Susana.

In: Plant Science, Vol. 253, 2016, p. 154-163.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity

AU - Alves Carvalho, Dalia

AU - Vasconcelos, Marta W.

AU - Lee, Sangseok

AU - Koning-Boucoiran, Carole F.S.

AU - Vreugdenhil, Dick

AU - Krens, Frans A.

AU - Heuvelink, Ep

AU - Pinto de Carvalho, Susana

PY - 2016

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N2 - High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.

AB - High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.

KW - Abscisic acid

KW - Stomatal anatomy

KW - Tetraploid roses

KW - Vapor pressure deficit

KW - Water loss

U2 - 10.1016/j.plantsci.2016.09.018

DO - 10.1016/j.plantsci.2016.09.018

M3 - Article

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SP - 154

EP - 163

JO - Plant Science

JF - Plant Science

SN - 0168-9452

ER -